China Ocean Mineral Resources R D Association [DY115-02-2-04]; Hi-Tech Research and Development of China [2007AA091407]; Marine Scientific Research Special Foundation [200805050]Strain WPAGA1(T) was isolated from marine sediment of the west Pacific Ocean. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belonged to the genus Flammeovirga. Strain WPAGA1(T) exhibited highest 16S rRNA gene sequence similarity with Flammeovirga yaeyamensis NBRC 100898(T) (98.1%) and lower sequence similarity with Flammeovirga arenaria IFO 15982(T) (94.6%) and other members of the genus Flammeovirga (<94.2%). DNA DNA relatedness studies showed that strain WPAGA1(T) was distinct from F. yaeyamensis NBRC 100898(T) and F. arenaria NBRC 15982(T) (43 +/- 4% and 32 +/- 2% relatedness values, respectively). Strain WPAGA1(T) could be distinguished from all known members of the genus Flammeovirga by a number of phenotypic features. However, the dominant fatty acids of strain WPAGA1(T) (iso-C-15:0, C-16:0 and C-20:4 omega 6,9,12,15c), the major polyamine (cadaverine) and the G+C content of the chromosomal DNA (32.9 mol%) were consistent with those of members of the genus Flammeovirga. Based on phenotypic and chemotaxonomic features and 16S rRNA gene sequences, strain WPAGA1(T) can be assigned to the genus Flammeovirga as a representative of a novel species, for which the name Flammeovirga pacifica sp. nov. is proposed; the type strain is WPAGA1(T) (=CCTCC AB 2010364(T)=LMG 26175(T)=DSM 24597(T)=MCCC 1A06425(T))
