Background and Aim : Brucella melitensis is one of the Important foodborne microorganisms . Especially in dairy products such as traditional cheeses. These microorganisms cause fever, sweating, headache, back pain and weakness. cheese such as milk is a nutrient-free product for the human and source of protein and calcium, The approximate amount of cheese production in Iran is 100,000 tons and based on the latest statistics, per capita consumer of 34 kg, and the traditional Iranian cheese is one of the most consumer milk fermented products in Iran, and depending on how it is proven to be different in different areas, sensory, physicochemical and microbial features are different. This research was aimed to Investigation of Contamination of brucella meletensis in traditional cheese produced in Qazvin in 2017-2018
Material and Methods : In this descriptive and analytical study, From october 2017 to september 2018, a total of 112 samples of traditional cheese were collected from different places in Qazvin. After collectimg the samples,they were transported to the laboratory. All isolates were subjected to biochemical test.Brucella melitensis strains were further confirmed by PCR amplification.Date were analyzed using Chi- square test and Fishers exact test. Statistical difference was considered significant (P<0/05).
Results: Of the 112 samples with standard culture methods were studied, 14 sample (12/5%) were positive, and looking at the reviews with positive samples from 14 molecular with culture, 7 samples (6/25%) reported a positive real. the most pollution in the white cheeses (5/35%) Was seen. It is also the most pollution in autumn(1/7 %) and winter(3/5 %) Was reported.
Conclusion: With regard to the pollution of the sample reviewed, pay attention to the safety and health of the traditional cheese production centers as well as to the prevention of the supply of the required subjects, it seems. As well as the use of molecular techniques to identify food microorganisms in terms of spending the time and money, and most importantly the accuracy of these methods, can be a good alternative to the slew of molecular methods.
Keywords: Brucella meletensis , traditional cheese, PC
