Construction and evaluation of in house pcrbased diagnostic method for detection of helicobacter pylori infection

Abstract

The specific primers for He/icobacter pylori were constructed. The primer were standardized and optimized by using ATCC strains. The PCR were performed to eighty-nine antral biopsy samples from patients suspected to have gastric ulcer. Simultaneously, histopathological examination was performed on the biopsy samples. The agreement between the two test methods was measured

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