Inhibition of PBS⁺⁺-induced lysosome spreading by MUC-G.

Abstract

HeLa cells were incubated for 30 min in RPMI containing 10% serum (R10) or in PBS++, in absence or in the presence of 40 μg/ml MUC-G or MUC-CL, and then processed for immunofluorescence analysis for detection of lysosome (green), actin (red) and nucleus (blue) by confocal microscopy. Scale bar = 20 μm. Note the PBS++-induced lysosome spreading, with accumulation of lysosomes at the cell edges (white arrows) and inhibition by MUC-G.</p