Antonissen, Gunther

Croubels, Siska

Daeseleire, Els

De Saeger, Sarah

Valgaeren, Bonnie

Van Pamel, Els

Ghent University Academic Bibliography

Van Pamel E., Antonissen G., Valgaeren B., Croubels S., Daeseleire E. (2014) A multi-mycotoxin UHPLC-MS/MS method for the detection, quantification and identification of mycotoxins in milk replacer. 36th Mycotoxin Workshop, 16-18 June, Göttingen, Germany, p. 63.  Analysis of 12 mycotoxins in calves’ milk replacer  by means of UHPLC-MS/MS  E. Van Pamel1, G. Antonissen2,3, B. Valgaeren4, S. De Saeger5, S. Croubels3, and E. Daeseleire1 1 The Institute for Agricultural and Fisheries Research, Technology and Food Science Unit, Brusselsesteenweg 370, 9090 Melle, Belgium, 2 Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium, 3 Department of Pharmacology, Toxicology and Biochemistry, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium, 4 Department of Large Animal Internal Medicine, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium, 5 Department of Bio-analysis, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, 9000 Ghent, Belgium  E-mail: els.vanpamel@ilvo.vlaanderen.be    In Belgium, veal calves are predominantly fed liquid milk replacers, based on powder milk and vegetable protein sources such as soy, corn and wheat. These ingredients may imply a risk of mycotoxin contamination. The aim of this study was to develop a multi-mycotoxin ultra-high pressure liquid chromatographic-tandem mass spectrometric (UHPLC-MS/MS) method for the detection, quantification and identification of 12 mycotoxins in milk replacer. The mycotoxins included in this study were aflatoxins (AFB1 and B2), alternariol (AOH), alternariol monomethyl ether (AME), deoxynivalenol (DON), 3-acetyl-DON (3-Ac-DON), 15-acetyl-DON (15-Ac-DON), fumonisins (FB1 and B2), ochratoxin A (OTA), T-2 toxin (T-2) and zearalenone (ZEN). 13C15-DON, 13C17-3-AcDON, 13C24-T-2 and 13C18-ZEN were used as internal standards.   Gradient chromatographic separation was performed on an AcquityTM UHPLC (Waters) system equipped with an Acquity CSH Fluoro Phenyl column (1.7 µm, 2.1 x 150 mm, Waters). The mobile phase consisted of a mixture of H2O + 0.3% acetic acid (A) and methanol (MeOH) + 0.3% acetic acid (B). Detection was performed with a Xevo TQ-S MS triple quadrupole system (Waters). The extraction procedure consisted of a first extraction step with MeOH followed by a second extraction step with acetonitrile/H2O/acetic acid (79/20/1). Specificity, linearity (R²), apparent recovery (RA), repeatability (RSDr), reproducibility (RSDR) , limit of detection (LOD) and quantification (LOQ) were determined for the method developed. For the calculations of RA, RSDr and RSDR two concentration levels were considered. For all mycotoxins considered, the criteria of linearity (R² ≥0.99) and specificity were fulfilled. The average RA (over the two concentration levels) varied between 85% and 107%, which is in agreement with the ranges stipulated in Commission Decision 2002/657/EC. Average RSDr and RSDR ranged between 0.9 and 10.5% and between 1.5 and 12.8%, respectively. The LOD values were within the range of 1-89 µg/kg and 2-171 µg/kg , respectively.  The developed method was used to determine the degree of mycotoxin contamination in different samples of calves’ milk replacer (Antonissen et al., 2014).  References Antonissen G., Valgaeren B., Detavernier C., De Saeger S., Van Pamel E., Daeseleire E., Pardon B., Ducatelle R., Deprez P., Croubels S. (2014) Assessment of mycotoxin occurence in feed samples from the veal calf industry. 36th Mycotoxin Workshop, 16-18 June, Göttingen, Germany, p. 123. 

A multi-mycotoxin UHPLC-MS/MS method for the detection, quantification and identification of mycotoxins in milk replacer

https://biblio.ugent.be/publication/5673798/file/5673800

A multi-mycotoxin UHPLC-MS/MS method for the detection, quantification and identification of mycotoxins in milk replacer

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Ghent University Academic Bibliography