本实验采用酶学和现场采样等方法研究日本囊对虾精巢发育过程中精巢与肝胰腺的形态学和几种生化组分含量的变化,旨在说明海区自然状态下日本囊对虾亲虾生殖过程营养物质在组织器官中的消长与转移的情况。结果表明,台湾海峡海区自然状态下日本囊对虾精巢发育过程中,精巢指数从I期0.010±0.001~IV期0.017±0.002呈逐渐上升(P0.05)。葡萄糖、胆固醇和甘油三酯的含量(Mg/dl)变化情况分别如下:在精巢中,为14.85±5.40~4.75±3.17、7.69±2.47~16.46±6.79和15.85±4.56~36.78±8.12;在肝胰腺中,为10.95±2.60~22.24±5.76、27.12±9.11~202.62±77.26和42.87±13.39~116.99±24.57;在血淋巴中,为6.38±1.44~11.25±3.08、53.77±32.10~155.32±62.55和13.67±7.06~79.56±38.19。本文还就对虾精巢发育过程中,性腺和肝胰腺的形态变化,以及肝胰腺、血淋巴、精巢中葡萄糖、胆固醇和甘油三酯的含量的消长与转移的情况进行了详细的讨论。In this study,enzymatic,field sampling and other methods were used to study pancreatic morphology and several biochemical component content changes in spermary and liver of Marsupenaeus japonicus during the spermary development.It intended to illustrate in the natural state,the growth and decline and transfer condition of nutrition substances in the tissues and organs of Marsupenaeus japonica broodstock in reproductive process.The results showed that in the natural state of the Taiwan Strait waters,Marsupenaeus japonicus,testis index from the phase I 0.010 ± 0.001--IV period of 0.017 ± 0.002 was gradually increased(P0.05).Glucose,cholesterol and triglyceride levels(mg/dl) changed as follows: In the testis,is 14.85 ± 5.40 -- 4.75 ± 3.17,7.69 ± 2.47--16.46 ± 6.79 and 15.85 ± 4.56--36.78 ± 8.12;in the liver pancreas,is 10.95 ± 2.60--22.24 ± 5.76,27.12 ± 9.11--202.62 ± 77.26 and 42.87 ± 13.39 -- 116.99 ± 24.57;in the hemolymph,is 6.38 ± 1.44--11.25 ± 3.08,53.77 ± 32.10--155.32 ± 62.55 and 13.67 ± 7.06 --79.56 ± 38.19.This paper also showed that during the development of Marsupenaeus japonicus spermary,how gonad、pancreas and liver changeed in morphological area.The situations of rise and fall with the transfer in liver,pancreas,blood,lymph,testis glucose,cholesterol and triglyceride were also discussed in detail.福建省科技计划重点项目(2008N0040)资
