A five-week study on the pathology of condemned livers of 100 pigs comprising 75 porkers and

25 sows at Shah A lam abattoir was conducted. The liver lesions consisted of36 with milk spots, 16 with

perihepatitiS, 12 with abscessations, 11 with cholangiohepatitzs, 8 with cholangitzs, 5 with nodular

hyperplasia, 4 with focal necrosis, 3 with post-necrotic scarring, 2 with liPidoszS and one case each of

cystic bile duct hyperplasia, massive necrosis and hepatocarcinoma. Milk spots due to migration of

Ascaris suum larvae was a major pro blem in porkers (41 %). Fourteen (39%) of the milkspot livers

were severely affected having a 4 + lesion score with all the lobes affected. PerihepatitIs was seen only

in porkers while nodular hyperplasia only in sows. Escherichia coli and Kliebsiella sp. were the

common bacteria Isolated from the abscesses. The possible causes of the lesions are discussed

Abdul Rahman, Sheikh Omar

Tahir, Siti Salmiah

Universiti Putra Malaysia Institutional Repository

Pertanika 8(2), 259 - 263 (1985)An Abattoir Survey on the Pathology ofSwine LiversSIn SALMIYAH TAHIR and A.R. SHEIKH-OMARFaculty of Veterinary Medicine and Animal Science,Universiti Pertanian Malaysia,Serdang, Selangor, Malaysia.Key words: Abattoir survey; pathology; swine liversABSTRAKSatu kajian patologi pada hati babi yang telah rosak dijalankan selama lima minggu di rumahsembelih Shah Alam. Satu ratus hati telah dikumpulkan, 75 danpadanya ialah babi 'porker' dan 25babi betina tua. Lesi-lesi yang dilihat ialah 36 'milkspots', 16 perihepatitis, 12 abses, 11 kolangiohepa-titzs, 8 kolangitzs, 5 hzperplasia nodular, 4 nekroszs, 3 'post necrotic scarring', 2 lzpidoszS dan satu'cystic bile duct hyperplasia', 'massive necroszS' dan hepatokasinoma. 'Milkspots' akibat danpada per-pindahan larva Ascaris suum adalah satu masalah besar terutama dalam babi porker (41%). Empatbelas (39%) danpada hati 'milkspot' diberikan gred lesi 4 + di mana semua lobanya terlibat.Pen'hepatitzS dilihat hanya dalam babi porker dan hzperplasia nodular hanya dilihat dalam babibetina tua. Escherichia coli dan Kliebsiella sp. adalah dua spesies bakteria yang kerap diasingkandaripada 12 abses. Penyakit-penyakit yang mungkin menyebabkan lesi-lesi yang dilihat dibincang-kan.ABSTRACTA five-week study on the pathology of condemned livers of 100 pigs comprzSing 75 porkers and25 sows at Shah A lam abattoir was conducted. The liver lesions conszSted of36 with milkspots, 16 withperihepatitzS, 12 with abscessations, 11 with cholangiohepatitzs, 8 with cholangitzs, 5 with nodularhyperplasia, 4 with focal necroszS, 3 with post-necrotic scarring, 2 with lzPidoszS and one case each ofcystic bile duct hyperplasia, massive necroszS and hepatocarcinoma. Milkspots due to migration ofAscaris suum larvae was a major pro blem in porkers (41 %). Fourteen (39%) of the milkspot liverswere severely affected having a 4 + lesion score with all the lobes affected. PerihepatitIs was seen onlyin porkers while nodular hyperplasia only in sows. Escherichia coli and Kliebsiella sp. were thecommon bacteria Isolated from the abscesses. The possible causes of the lesions are dzScussed.INTRODUCTIONThe liver is the principal organ of meta-bolism for many endogenous and exogenoussubstances and as a result is one of the mostfrequently damaged organs in the body. It has alarge capacity to regenerate in response to injuryand as a result livers of clinically healthy animalsshow a spectrum of disease conditions atslaughter. In a study of 8,558 swine from anabattoir in Malaysia, the causes of condemna-tions of livers comprised 0.12% with milkspots,0.06% with cirrhosis, 0.05% with fatty change,0.02% with abscesses and 0.01 % with otherchanges (Tham and Sheikh-Omar, 1981). Inanother study, the majority (71.4%) of livers of5,466 swine was condemned due to milkspots(Yap et al., 1983).This paper reports a survey on the patho-logy of livers of pigs slaughtered at the ShahAlam abattoir near Kuala Lumpur.SITI SALMIYAH TAHIR AND A.R. SHEIKH·OMARMATERIALS AND METHODSOne hundred condemned livers of pigsslaughtered at Shah Alam abattoir were collect-ed on several visits to the abattoir in May andJune, 1984. Most of the pigs slaughtered hadcome from Selangor and Negeri Sembilan andoccasionally from Province Wellesly and Perak.Each liver was examined for gross abnor-malities. From each liver tissue, samples weretaken, one each from the four main lobes andfrom any area that showed pathological changes.The specimens were fixed in 10% neutral buffer-ed formalin and later embedded in paraffin waxand 4 J.l m sections were stained with haemato-xylin and eosin and where necessary, selectedsections were also stained by the Masson'strichome method for connective tissue and theGram's stain for gram positive and negativebacteria.The surface of all abscesses present waslanced and the pus sampled aseptically withcotton swabs and cultured on blood agar andMacConkey agar aerobically at 37°C for 24hours and anaerobically in an anaerobicchamber at 37°C for 48 hours. The organismsisolated were identified based on Gram reaction,microscopic morphology, colonial characteristicsas well as standard biochemical techniques(Cowen, 1977).RESULTSOf the 100 condemned livers examined, 75were from porkers and 25 were from sows. Acombination of lesions was present in most livers.The most predominant lesions seen in each liverare presented in Table 1. The common lesionswere milkspots (36%), perihepatitis (16%),abscessations (12%) and cholangiohepatitis(11 %). In porkers, the most common lesionswere milkspots (41 %) and perihepatitis (21 %)while in sows, cholangiohepatitis (20%), nodularhyperplasia (20%) and milkspots (20%) weremost common.TABLE 1The most prominent lesions observed in condemned livers*Number of liversLesion Porker Sow TotalMilkspots 31 5 36Perihepatitis 16 16Abscessation 9 3 12Cholangiohepatitis 6 5 11Cholangitis 7 8Nodular hyperplasia 5 5Focal hepatic necrosis 3 4Post necrotic scarring 2 3Hepatic lipidosis 2 2Cystic bile duct hyperplasiaMassive necrosisHepatocarcinomaTotal 75 25 100*a combination of lesions was observed in most livers.260 PERTANlKA VOL. 8 NO.2, 1985AN ABATTOIR SURVEY ON THE PATHOLOGY OF SWINE LIVERSTABLE 2The severity of milkspot lesions in 36 pigsMilkspots were the most common lesionsseen. Grossly, they were small stellate whitespots, 0.1 cm to 1.5 cm in diameter. In a fewlivers, the bigger spots tended to have a nodularcystic centre of 0.1 cm diameter. The rightlateral lobe and left and right medial lobes weremost commonly affected. The lesions scoredaccording to the number of lobes affected areshown in Table 2 and most livers had a score of4 + in which all the lobes were affected. Micros-copically, there was mild to marked focalfibrosis, biliary hyperplasia and cellular infiltra-tion with predominantly eosinophils andlymphocytes.TABLE 3Bacteria isolated from 12 hepatic abscessesbacteria isolated in mixed cultures and theirisolation frequency are shown in Table 3. Themost common isolates were Escherichia coliand Kliebsiella sp. One abscess each was positivefor Salmonella sp. and Pseudomonas pseudo-mallei.75322Frequency of isolation*Escherichia coliKliebsiella spStaphylococcus aureusCitrobacter freundiiProteus sp.Corynebacterium pyogenesCitrobacter intermediusSpeciesNumber of livers affectedLesion score*4+3+2+1+148311Pseudomonas pseudomalleiProteus rettgeriSalmonella sp.*in mixed culturesTotal 36*4 + - all lobes affected3 + - three lobes affected2 + - two lobes affected1+ - one lobe affectedPerihepatitis was seen in 16 animals (16%),all of which were porkers. The affected livershad thickened and cloudy capsule, sometimeswith fibrin tags causing fusion of the lobes andadhesion to the diaphragm. In severe cases, thefusion was complete and the liver appeared asone large mass. One liver was severely affectedand adhered to the diaphragm. Microscopically,there was capsular and subcapsular fibrosis.Abscessation was seen in 12 animals com-prising 9 porkers and 3 sows. The abscessesranged from 0.1 cm to 1. 5 cm in diameterappearing as one focus or multifoci and affectingall lobes. The abscesses contained caseated,creamy whitish to greenish yellow material whichwas sometimes dry and calcified. The species ofCholangiohepatitis was diagnosed micros-copically in 11 animals (11 %) comprising 6porkers and 5 sows. Histopathological findingsincluded fibrous tissue replacement of liverlobules, biliary hyperplasia and periportal infil-tration by lymphocytes and eosinophils. Eightlivers had cholangitis, 7 from porkers and onefrom a sow. Histologically, mild to moderatefibrosis with marked eosinophil infiltration inthe portal triads were present.Nodular hyperplasia was observed in sowsonly. The affected livers had a few firm noduleswhich were reddish on the cut surface andseparated from normal liver parenchyma by athin capsule. Histologically, the nodules consist-ed of normal hepatocytes but without the normallobular architecture and were surrounded by athin layer of fibrous tissue.Four livers had focal hepatic necrosis,microscopically seen as focal areas ofhepatocytesundergoing pyknosis, karyorrhexis and karyoly-PERTANIKA VOL. 8 NO.2. 1985 261SITI SALMIYAH TAHIR AND A.R. SHEIKH·OMARsis. Post necrotic scarring was seen in 3 liverswhich had typically marked periportal andportal fibrosis as well as distortion of hepaticlobules.One case each of cystic bile duct hyper-plasia, massive necrosis and hepatocarcinomawere also observed in sows. The first had cystlikedilations of bile duct with hyperplastic cuboidalepithelium. Massive necrosis was seen in a brownand friable liver which histologically revealednecrosis of hepatocytes of entire lobules whichwere filled with erythrocytes. The liver withhepatocarcinoma was enlarged and had a singleraised nodule which appeared homogenouslybrown and multilobulated on the cut surface.Histologically, there was loss of normal liverarchitecture with neoplastic cells present as solidmasses separated by connective tissue septa. Thecells were large round with prominent round toovoid hyperchromatic nuclei and had noresemblence to normal hepatocytes.DISCUSSIONThe high frequency of livers with milkspotsin this study is consistent with the findings ofother workers (Bottle et al., 1975; Polley andMostert, 1980). This means that infection withthe causative agent Ascaris suum is a problem inporkers in Malaysia and is most likely due toimproper deworming schedules and poor sani-tary measures at the farms. However, work doneoverseas has shown that A. suum infection stillapproaches 100 percent despite widespread useof anthelmintics and confined housings (Froe II,1982). Egg of A. suum are very resistant to harshenvironmental conditions and have been foundto remain viable in manure collection pits up to14 months (Smith, 1979). Untreated, infectedpigs act as an important source of infection toother pigs by shedding 'infective' eggs when theyreach 8 weeks of age thus contaminating thestalls (Froe II, 1982).The pigs studied were clinically healthy.They were infected with A. suum probably at agrower phase and were able to acquire immunityagainst the parasite. Had the infection develop-ed during the prestarter and starter phases, highmortalities and growth retardation would havebeen prominent (Froe II, 1982). Segments ofascaris larvae and aggregations of lymphoid cellswere not observed in this study because thelesions had been going on for some time. Suchsegments have been detected 21 days post infec-tion with ascaris (Copeman and Gaafar, 1972).It was interesting to find perihepatitis in thisstudy but the cause has remained unestablished.Copeman and Gaafar (1972) described the possi-bility of ascarid larval migration causing cloud-ing of the capsule 24 hours after infection andprogressing to opacity by the 12th day. Myco-plasma hyorhinzs and Haemophilus parasuzs arethe other possible causes (Leman et at., 1981). Inthis study, all the affected livers had markedeosinophil infiltration suggestive of parasiticinvolvement.The isolation of bacteria from the liverabscesses in mixed cultures mainly consisting ofEscherichia coli, Kliebsiella sp. and Staphyloc-cus aureus are similar to the findings of otherworkers (McCracken and McCaughey, 1973;Engvall and Schwan, 1983). The one liverabscess due to Pseudomonas pseudomallei hadtypical microscopic lesions of melioidosis (Omar,1963; Thomas et at., 1981).Nodular hyperplasia which was seen in sowswas consistent with the findings of Hayashi et at.(1983) who reported an incidence of 40 permillion pigs and suggested hepatocarcinogens asa possible cause.Hepatocarcinoma was the only neoplasmseen. Its occurence is low in pigs, occuring at therate of one per 5.5 million pigs (Moulton, 1978).Hepatic lipidosis as seen in this study hasbeen reported by Yap et al. (1983) at a higherfrequency (11.9%). This problem usually ariseswhen the fat is mobilised too rapidly fromvarious fat depots when the animal is under-nourished Oubb and Kennedy, 1970). It is alsoseen in pigs suffering from severe protein mal-nutrition causing great reduction in hepaticphospholipid and disturbance in lipid meta-bolism (Gupta, 1973).262 PERTANIKA VOL. 8 NO.2, 1985AN ABATTOIR SURVEY ON THE PATHOLOGY OF SWINE LIVERSThe source of the pigs studied could not betraced due to complete lack of history and henceepidemiological inferences could not be made.However the data presented do provide furtherinformation on the spectrum of disease condi·tions occuring in livers of slaughtered pigs.ACKNOWLEDGEMENTSWe wish to thank the laboratory staffmembers of the Department of VeterinaryPathology and Microbiology, Faculty of Veteri-nary Medicine and Animal Science for technicalassistance and Ms. Aminah Ibrahim for typingthe manuscript.REFERENCESBOTTLE. E.G., MCLAMB. R.D., VESTAL. T.J. (1975):Swine Parasites: Causes of liver condemnations.Vet. Med. Small Anim, Clin. Agric. Practi., 70:809-813.COPEMAN. D.B. and GAAFAR. S.M. (1972): Sequentialdevelopment of hepatic lesions of ascaridiosis incolostrum-deprived pigs. Aust. Vet.]., 48: 263-268.COWAN. S.T. (1977): Cowan and Steel's Manual forthe identification of medical bacteria,Cambridge, University Press.ENGVALL. A. and SCHWAN. O. (1983): Isolation andcharacterisation of bacteria recovered fromabscesses of normal slaughtered pigs. Act. Vet.Scand., 24: 74-83.FROE II, D.L. (1982): Ascarid larval migration. Med.Vet. Pract., 63: 829 - 832.GUPTA. P.P. (1973): A study on quantitive changes inliver lipids of Indian pigs suffering from severeprotein malnutrition. Indian]. Anim. Sci., 43:354- 858.HAYASHI. M., TSUDA. H., ITO. N. (1983): Histo-pathological classification of spontaneous hyper-plastic liver nodules in slaughtered swine. ].Compo Path. 93: 603 - 612.JUBB. K.V.F. and KENNEDY. P.C. (1970): Pathologyof Domestic Animals. Vol. II, 2nd ed. New York,pp. 191- 261. Academic Press.LEMAN. A.D., GLOCK. R.D., MENGELING: W.L.,PENNY. R.H.C., SCHOLLE. E., STRAW. B. (1981):Diseases of swine. 5th ed. Ames, Iowa, The IowaState University Pres, USA.MOULTON.J.E. (1978): Tumors in Domestic Animals.2nd ed. Revised University of California PressBerkely, Los Angeles, London. pp. 273 - 287.Mc CRACKEN. A. and Mc CAUGHEY. W-J. (1973):A survey of abscesses in bacon weight pigs. Br.Vet.]., 129: 359-361.OMAR. A.R. (1963): Pathology of melioidosis in pigs,goats and horse.]. Camp. Path., 73: 359 - 373.POLLEY. L.R. and MOSTERT. P.E. (1980): Ascarissuum in Saskatchewan pigs: An abattoir survey ofprevalence and intensity of infection. Can Vet.].,21: 307 - 309.SMITH. J.P. (1979): Viability of larvated swine swineascarid (Ascaris suum) eggs in a conventionalnon-aerated manure collection pit. Southwest.Vet., 32: 33 - 35.THAM. K.M. and SHEIKH-OMAR. A.R. (1981): A studyon causes of condemnation of carcass and organsat Shah Alam abattoir. Pertanika, 4: 43 - 46.THOMAS. A.D., NORTON. J.H., FORBES. J.C.,FAULKNER (1981): Melioidosis in an intensivepiggery. Aust. Vet.]., 57: 144-145.YAP. T.C., MOHAN. S.S. and GILL. H.S. (1983): Astudy on causes of condemnation of carcass andorgans of livestock slaughtered at the Shah Alamabattoir. Kajian Vet., 15: 1-10.(Received 22 March, 1985)PERTANIKA VOL. 8 NO.2. 1985 263

An Abattoir Survey on the Pathology of Swine Livers

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An Abattoir Survey on the Pathology of Swine Livers

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