Acknowledgments This work was supported by funding from the National Institute of General Medical Sciences of the National Institutes of Health, Number: P30GM114750 and Oh–Zopfi Grant for Perinatal Research, Department of Pediatrics; Kilguss Research Core at Women & Infants Hospital of Rhode Island. Funding information National Institute of General Medical Sciences, Grant/Award Number: 30GM114750; Oh–Zopfi Award for Perinatal Research, Women & Infants Hospital of Rhode IslandPeer reviewedPostprin

Abu-Eid, Rasha

Ahsan, Nagib

Najrana, Tanbir

Noble, Lelia

Sanchez-Esteban, Juan

Tollefson, George

Uzun, Alper

Pediatric Pulmonology

English

PubMed

Severe oligohydramnios (OH) due to prolonged loss of amniotic fluid can cause pulmonary hypoplasia. Animal model of pulmonary hypoplasia induced by amniotic fluid drainage is partly attributed to changes in mechanical compression of the lung. Although numerous studies on OH-model have demonstrated changes in several individual proteins, however, the underlying mechanisms for interrupting normal lung development in response to a decrease of amniotic fluid volume are not fully understood. In this study, we used a proteomic approach to explore differences in the expression of a wide range of proteins after induction of OH in a mouse model of pulmonary hypoplasia to find out the signaling/molecular pathways involved in fetal lung development. Liquid chromatography-massspectromery/mass spectrometry analysis found 474 proteins that were differentially expressed in OH-induced hypoplastic lungs in comparison to untouched (UnT) control. Among these proteins, we confirmed the downregulation of AKT1, SP-D, and CD200, and provided proofof-concept for the first time about the potential role that these proteins could play in fetal lung development

PubMed Central

Proteomic analysis of a murine model of lung hypoplasia induced by oligohydramnios

Acknowledgments This work was supported by funding from the National Institute of General Medical Sciences of the National Institutes of Health, Number: P30GM114750 and Oh–Zopfi Grant for Perinatal Research, Department of Pediatrics; Kilguss Research Core at Women & Infants Hospital of Rhode Island. Funding information National Institute of General Medical Sciences, Grant/Award Number: 30GM114750; Oh–Zopfi Award for Perinatal Research, Women & Infants Hospital of Rhode IslandPeer reviewe

Aberdeen University Research

Proteomic analysis of a murine model of lung hypoplasia induced by oligohydramnios

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Aberdeen University Research