Abone, M.

Fantahun, T.

Meseret, Selam

Mohammed, S.

Mrode, Raphael A.

Okeyo Mwai, Ally

Zewdie, G.

Zewdie, Yihenew

CGSpace

Genome Wide Association Study and Factors affecting Semen Volume and Concentration in Ethiopian Dairy Bulls Selam Meseret1*, Seid Mohammed2, Tegegn Fantahun2, Genet Zewdie2, Megersa Abone2, Genet Dejene2, Raphael Mrode1 and Okeyo A. Mwai11: International Livestock Research Institute2: Ethiopian Biotechnology Institute* s.meseret@cgiar.orgIntroductionDairy producers are demanding to the most fertile bull/semen. Selection of mature bulls to be sire of many progenies ispracticed based on the phenotypic information of semen quality parameters. Semen quality traits are complex traitscontrolled by genetic and environmental factors. Therefore, the aim of the study was to evaluate the effect of breed,ejaculation number, origin of bulls, age, and artificial insemination centers and perform genome wide associationunderlying semen volume and concentration.Methods• Retrospective data collected from five AI centers• 66 bulls genotyped using the Illumina BovineSNP50 BeadChip. • Covariate adjusted: Breed (HF= Holstein Friesian, JE= Jersey, & FFB= Holstein Friesian Borena cross), AI center (AIC, five centers), origin (seven origins), number of insemination (NE) and age ((<2, 2 to 3, 3 to 4, 4 to 5, and >5 years) for volume & concentration.• Mixed model used to estimate additive effects of SNP:α̂  = X*b + Z*g + e*• Significance tested (H0:gi = 0 vs. H1:gi ≠ 0, where H is the hypothesis) of ith SNP effects in g, the Wald test (W)ResultsSNP association with sperm volume ESAP  Conference, October 28-30, 2021, Addis Ababa, Ethiopia Conclusion: Identifying significantly associated SNPs with sperm quality traits helps to select candidatebulls for AI centers and decision could be make at early age.Figure 1. Effects of fixed factors on sperm volume and concentrationSperm volume and concentrationare affected significantly (p <0.001)by breed, artificial insemination,origin, number of ejaculations andage of the bulls.Principal component analysis Significant SNP, -Log10 P value > 5SNP association with sperm Concentrationrs134410301 7:32579149rs109814724 15:3264476rs110956165 15:3276723rs136532513 27:7091908rs109497662 27:7125956                rs110788394 27:6955584This document is licensed for use under the Creative Commons Attribution 4.0 International Licence. October 2021.

Genome wide association study and factors affecting semen volume and concentration in Ethiopian dairy bulls

https://cgspace.cgiar.org/bitstream/10568/116135/1/ESAP_poster_Selam.pdf

Genome wide association study and factors affecting semen volume and concentration in Ethiopian dairy bulls

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