Deshpande, S.P.

Gupta, R.

Jones, Christopher S.

Naravula, J.

Prasad, K.V.S.V.

Rathore, A.

Sivasubramani, S.

Somegowda, V.K.

Vemula, A.

Prasad, Kodukula V.S.V.

CGSpace

Fodder quality comparison in two sorghum populations under droughtVinutha K Somegowda1, 3, Prasad KVSV2, Jalaja Naravula3, Anilkumar Vemula1, Sivasubramani S1, Abhishek Rathore1, Chris S Jones2, Rajeev Gupta1 and Santosh P Deshpande11 International Crops Research Institute for the Semi-arid Tropics (ICRISAT)-HQ, Patancheru-502324, TS, India, 2 International Livestock Research Institute (ILRI), ICRISAT Campus, Patancheru-502324, TS, India, 3 VFSTR, Guntur, IndiaCONCLUSIONSConcurrent improvement of dry weight and IVOMD in sorghum crop improvement is possibleA gene Sobic.001G356000, linked to lignin and metabolizable energy under stress conditions was identified as a putative candidate geneSimilarly, the gene Sobic.001G463900 was linked to in vitro organic matter digestibilityDevelopment of traits linked SNPs for breeding applications and  fine mapping for IVOMD on chromosome 1 and 2 that will improve trait dissection is proposedBACKGROUNDDigestibility and lignin content determines feed quality and plant fitness and are inversely relatedA 5% variation in fodder digestibility is reported to result in a 20% price variation (Fig 1)Sorghum is a dual-purpose crop grown in Asia and AfricaCompared to maize, it is drought tolerant and has high water use efficiencyRESULTSMETHODSA recombinant inbred line (RIL) population (n=320, cross betweenICSV1×ICSV700) and a subset from reference collection (n=130) wereevaluated for terminal drought stress by withholding irrigation at boot leafstage for both populationsDry weight (DW) was recorded at maturity and the fodder was subjected tonear infrared spectroscopy (NIRS) to record, nitrogen content on dry matterbasis (NDM); neutral detergent fibre (NDF); acid detergent lignin (ADL);metabolizable energy (ME) and; in vitro organic matter digestibility (IVOMD).ICIM software and rMVP (R package) were used to identify genomic regionsassociated with traits under studyCandidate genes from QTL mapping and significant SNPs from GWAS wereidentified by annotation with the reference genome and Zea mays inPhytozome and synteny plots generated in CircosTable 1: Correlation for fodder quality traitsFig 1: Relation between price and fodder qualityFig 2b:MTAs based on Reference set and synteny with Zea mays Pearson’s correlation across years and treatments for DW and IVOMD, showednegative correlation with NDF and ADL (Table 1)While positive correlations were observed between DW, ME and IVOMD in theRIL populationHowever, in the reference set there was no strong positive or negativecorrelation between DW, ME and IVOMDSobic.008G026400 and Sobic.010G229000 were identified for the RILpopulation while Sobic.001G530300 and Sobic.008G054500 were identified inthe reference set under drought stress for IVOMDSobic.001G354800 was found to be associated with ADL, IVOMD and MEunder drought stress, which is associated with seed - dry grain maturityDry weight in the RIL population was associated with Sobic.007G145600 and Sobic.007G146200 (both on chromsome7) involved in multiple pathwayssuch as cell wall-bound phenolic acids that play a major role in plant defense against pathogensME in the reference set under stress was found to be associated with Sobic.003G282600 gene linked to homogalacturonan biosynthesis with a role in theplant cell wall that contributes to plant growth and development and cell wall structureThe syntenic relation between sorghum and maize showed that Sobic.007G023400 gene for IVOMD had 97.2% similarity with the maize geneGRMZM2G134134_T02, while Sobic.009G206700 for DW exhibited 98.6% similarity with the maize gene GRMZM2G319747_T02 (Fig 2)Pop Trait NDF ADL ME IVOMDREFDW 0 0.04 0.07 0.07NDF 0.60** -0.71** -0.78**ADL -0.75** -0.75**ME 0.97**RILDW -0.07 -0.14* 0.28** 0.19**NDF 0.60** -0.70** -0.75**ADL -0.79** -0.79**ME 0.97**Fig 2a: RIL population-based QTLs and synteny with Zea mays 

Fodder quality comparison in two sorghum populations under drought

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Fodder quality comparison in two sorghum populations under drought

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