A report (Rolinson and Stevens, 1961) on 6 D aminophenylacetamido penicillanic acid Penbdtin and its action on Gram-negative bacteria made us think it would be useful to test its activity on Brucella micro-organisms. This was done on 8 strains of Brucella melitensis all recently isolated in Malta, 5 of human and 3 of caprine origin. Parallel tests were also carried out with tetracycline hydrochloride and with chloramphenicol. Penicillin G was known to have little or no action on Brucella but was also tested for the sake of comparison.peer-reviewe