Gelatin is the denaturation product of the protein collagen. The denaturation can be performed by acid hydrolysis or base hydrolysis rendering A- type or B-type gelatin respectively. Gelatin molecules have a nonuniform distribution of 18 amino acids. As a typical protein, gelatin carries both positive and negative charges. Table 1 lists the main amino acids present in gelatin that can contribute towards the presence of a charge on a gelatin macromolecule, together with their respective pKa's.peer-reviewe