An infectious avian encephalomyelitis virus (IAEV) was isolated and characterized by physico—chemical and serological tests as well as by the disease and histological lesions it caused in chickens and chicken embryos. The behaviour of
two viruses, the NSW-l isolate and the van Roekel strain of IAEV in chickens, chicken embryos and tissue cultures were studied. Both viruses could be titrated in chicken embryos. The viruses propagated in chicken embryo neuroglial, chicken embryo kidney, chicken embryo fibroblasts and chicken embryo intestinal cell cultures, but failed to produce a cytopathogenic effect. Replication of IAEV was not detected in tracheal or intestinal organ cultures
