'Faculty of Veterinary Medicine, University of Zagreb'
Doi
Abstract
The evolution of cutaneous open wounds was evaluated in five client-owned dogs treated with photodynamic therapy (PDT), mediated by methylene blue and phototherapy with light-emitting diodes (LED) as the light source. On the first day of treatment, the wound was irrigated with sterile 0.9% sodium chloride solution, the surface was dried, and 3% solution of hydrogen peroxide soaked for 1 minute. The hydrogen peroxide was mechanically removed and, immediately after, 0.01% methylene blue was used in the wound for five minutes, after which a cluster of red LEDs (650 - 670 nm wavelength, 350 mW power, and irradiance of 200 mW/cm2) was used. Subsequently, single phototherapy sessions, every 48 hours or more, were applied until wound healing. In general, 24J was used for PDT and 12J for phototherapy. In all cases, the cutaneous open wounds treated with PDT associated with phototherapy healed without complications, and it may be considered as a treatment option, especially for extensive lesions.U istraživanje je uključeno pet pasa s poznatim vlasništvom kod kojih je procijenjeno cijeljenje otvorenih kožnih rana. Psi su liječeni fotodinamičkom terapijom (PDT) uz primjenu metilnog modrila i fototerapijom uz primjenu svjetlećih dioda (LED) kao izvora svjetla. Prvi dan pokusa rana je tretirana sterilnom 0,9 %-tnom otopinom natrijeva klorida, njezina je površina posušena te je tijekom jedne minute vlažena 3 %-tnom otopinom hidrogen-peroksida. Odmah potom hidrogen-peroksid mehanički je uklonjen, zatim je tijekom pet minuta upotrijebljeno 0,01 %-tno metilno modrilo, a nakon toga više povezanih crvenih LED dioda (valne dužine 650 – 670 nm, snage 350 mW i zračenja 200 mW/cm2). Poslije toga primijenjena je fototerapija, svakih 48 sati ili u duljem intervalu, sve dok rane nisu zacijelile. Primijenjeno je 24 J za PDT i 12 J za fototerapiju. U svim su slučajevima otvorene kožne rane, koje su liječene kombinacijom PDT-a i fototerapije, zacijelile su bez komplikacija. Navedeno otvara mogućnosti primjene ovih metoda, posebice na ranama s velikim lezijama