Four Aspergillus sp. strains were isolated from contaminated soil in Rio Grande, Southern Brazil. The biodegradation potential of these strains was evaluated using a simple method involving the determination of colony growth rates on plates containing a specific hydrocarbon or petroleumderivative as the only carbon source. The LEBM1 strain presented a high tolerance level to BTX. It was the only strain capable of growth on all the media, with growth rates varying from 1.3 to 2.2 mm/day. The LEBM2 strain presented the potential for phenol degradation, while the LEBM3 strain could be used for gasoline, diesel oil, hexane and chlorobenzene