Antuofermo, Elisabetta

Burrai, Giovanni P.

Caffara, Monica

Giorgi, Ilaria

Hetzel, Udo

Nuvoli, Sara

Pais, Antonio

Pedron, Claudio

Prearo, Marino

Rocca, Stefano

BMC Veterinary Research

PubMed

Elisabetta Antuofermo

Antonio Pais

Sara Nuvoli

Udo Hetzel

Giovanni P Burrai

Stefano Rocca

Monica Caffara

Ilaria Giorgi

Claudio Pedron

Marino Prearo

Springer - Publisher Connector

Mycobacterium chelonae associated with tumor-like skin and oral masses in farmed Russian sturgeons (Acipenser gueldenstaedtii)

Crossref

Helsingin yliopiston digitaalinen arkisto

The field vole (Microtus agrestis) is a known maintenance host of mycobacterium microti. Previous studies have shown that infected animals develop tuberculosis. However, the disease is also known in cats and is sporadically reported from humans and other mammalian species. We examined trapped field voles from an endemic area, using a range of diagnostic approaches. These confirmed that a combination of gross and histological examination with culture is most appropriate to identify the true prevalence of the disease, which was shown to be more than 13% at times when older animals that have previously been shown to be more likely to develop the disease dominate the population. The thorough pathological examination of diseased animals showed that voles generally develop systemic disease with most frequent involvement of spleen and liver, followed  by skin, lymph nodes, and lungs. The morphology of the lesions was consistent with active disease, and their distribution suggested skin wounds or oral and/or aerogenic infection as the main portal of entry. The demonstration of mycobacteria in open skin lesions, airways, and salivary glands indicated bacterial shedding from the skin and with sputum and saliva. This suggests not only the environment but also direct contact and devouring as likely sources of infection

Antuofermo, E

Pais, A

Nuvoli, S

Hetzel, U

Burrai, G P

Rocca, S

Caffara, M

Giorgi, I

Pedron, C

Prearo, M

ZORA

Non-tuberculous mycobacteria responsible for piscine mycobacteriosis usually produce visceral granulomas in both freshwater and marine species. In this study, the first occurrence
of Mycobacterium chelonae associated with tumor-like lesions in the Russian sturgeon
(Acipenser gueldenstaedtii) is reported. Fifteen sturgeons from an Italian fish farm showing
skin and oral cauliflower-like masses were investigated by histopathology, bacterial culture
and molecular analyses.
A total of 20 masses different in size located in the mouth and in pectoral and caudal fins
(characterized by abundant calcium deposits and by mild to moderate granulomatous
inflammation) were observed with a significant different degree of histological severity. All
internal organs of the fish were negative for mycobacteria, Ziehl-Neelsen was positive in only
one of the oral masses, whereas bacterial and PCR analyses detected the presence of M.
chelonae for almost all the skin and oral masses. Based on these results, a calcinosis of
dystrophic origin associated with a chronic granulomatous inflammation was considered as a
primary diagnosis consequent to tissue injury in areas susceptible to trauma.
We hypothesized that the occurrence of M. chelonae in farmed sturgeons was only a
secondary event related to its presence in a stressful rearing environment and subsequent to a
dystrophic calcinosis occurred in previously damaged tissues

Antuofermo E.

Pais A.

Nuvoli S.

Hetzel U.

Burrai G. P.

Rocca S.

CAFFARA, MONICA

Giorgi I.

Pedron C.

Prearo M.

Archivio istituzionale della ricerca - Alma Mater Studiorum Università di Bologna

English

Mycobacterium chelonae associated with tumor-like skin and oral masses in
farmed Russian sturgeons (Acipenser gueldenstaedtii)

Background: Non-tuberculous mycobacteria responsible for piscine mycobacteriosis usually produce visceral granulomas in both freshwater and marine species. In this study, the first occurrence ofMycobacterium chelonaeassociated with tumor-like lesions in the Russian sturgeon (Acipenser gueldenstaedtii) is reported. Fifteen sturgeons from an Italian fish farm showing skin and oral cauliflower-like masses were investigated by histopathology, bacterial culture and molecular analyses.Results:A total of 20 masses different in size located in the mouth and in pectoral and caudal fins (characterized by abundant calcium deposits and by mild to moderate granulomatous inflammation) were observed with a significant different degree of histological severity. All internal organs of the fish were negative for mycobacteria, Ziehl-Neelsen was positive in only one of the oral masses, whereas bacterial and PCR analyses detected the presence ofM. chelonaefor almost all the skin and oral masses. Based on these results, a calcinosis of dystrophic origin associated with a chronic granulomatous inflammation was considered as a primary diagnosis consequent to tissue injury in areas susceptible to trauma.Conclusions:We hypothesized that the occurrence ofM. chelonaein farmed sturgeons was only a secondary event related to its presence in a stressful rearing environment and subsequent to a dystrophic calcinosis occurred in previously damaged tissues

Burrai, Giovanni Pietro

CINECA IRIS Institutial research information system UNISS

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Mycobacterium chelonae associated with tumor-like skin and oral masses in farmed Russian sturgeons (Acipenser gueldenstaedtii)

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Helsingin yliopiston digitaalinen arkisto

ZORA

Archivio istituzionale della ricerca - Alma Mater Studiorum Università di Bologna

Springer - Publisher Connector

CINECA IRIS Institutial research information system UNISS