A two-frequency dual-plane stereo particle image velocimetry (DSPIV) technique is described for highly resolved measurements of the complete nine-component velocity gradient tensor field ∂ u i /∂ x j on the quasi-universal intermediate and small scales of turbulent flows. The method is based on two simultaneous, independent stereo particle image velocimetry (PIV) measurements in two differentially spaced light sheet planes, with light sheet characterization measurements demonstrating the required sheet thicknesses, separation, and two-axis parallelism that determine the measurement resolution and accuracy. The present approach uses an asymmetric forward–forward scatter configuration with two different laser frequencies in conjunction with filters to separate the scattered light onto the individual stereo camera pairs, allowing solid metal oxide particles to be used as seed particles to permit measurements in nonreacting as well as exothermic reacting turbulent flows.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/47075/1/348_2004_Article_898.pd