A new methodology is presented for molecular phylogenetic analysis addressing a fundamental problem in biology, name the reconstruction of the Tree of Life (TOL). Here, phylogenies are based on patterns of hybridization similarity in their DNA. Furthermore, phylogenies are based on a set of universal biomarkers (so-called nxh chips) chosen a priori, independently of the target group of organisms. Therefore, this methodology enables analyses of groups with biologically distant organisms, hence could be scaled to obtain a universal tree of life. Unlike conventional molecular methods, it produces a hypothesis in a single run, without optimizing across numerous hypotheses for consensus. Prototype hypotheses agree with the biological Ground Truth in over 70% of the relationships. Higher quality nxh chips are likely to produce better hypotheses, but more difficult to design
