This study aimed at investigating the tilapia fish (Oreochromis Niloticus) DNA. In analyzing Tilapia DNA, DNA extraction was carried out and PCR mixture was used for 20 μL of reaction, making Master Mix such as 1 μL Forward Primer, 1 μL Reverse Primer, 10 μL HotStar Taq Master Mix, 1 μL DNA Sample, 7 μL Nuslease Free Water. Mixing is done by adding the enzyme at the last stage. Then turn on the thermal cycler and set it according to the desired PCR conditions. Electrophoresis is carried out through stages such as Pre-Denaturation, Denaturation, Annealing, Extention, Final extension. The results showed that the virus did not infect tilapia (Oreochromis Niloticus). Tilapia sprayed on the gills with various doses showed the same results, namely no detection of KHV in mucus, kidneys, and liver
