The study utilized an experimental method of research. Randomized complete block design was employed in assigning variables and gathering data. Cymbopogon citratus was collected from Dasmariñas, Cavite, Philippines. Extracted citral from lemon grass and bacteria were obtained from Department of Science and Technology-Industrial Technology Development Institute (DOST-ITDI), Taguig, Metro Manila, Philippines. Measuring the bacterial growth and zone of inhibitions compared the antibacterial activity of Cymbopogon citratus citral leaf extract against bacterial pathogens. Based on the results, Cymbopogon citratus leaf essential oil extract showed complete inhibition activity against Enterobacter aerogenes and Escherichia coli. The positive controls, Meropenem and Cefuroxime also showed complete inhibition activity against Enterobacter aerogenes and Escherichia coli. The positive controls, Meropenem and Cefuroxime also showed complete inhibition of Enterobacter aerogenes and Escherichia coli respectively. Cefuroxime showed severe reactivity against the organism, with the inhibitory zone extended 10 mm beyond the specimen. The negative controls, sample free discs, yielded no detectable zone of inhibitions against bacteria. Therefore, Cymbopogon citratus leaf essential oil (citral) had complete inhibitory activity against Eschirichia coli and Enterobacter aerogenes. It had slightly wider zone of inhibition in Escherichia coli (12.22mm) compared to the zone of inhibition in Enterobacter aerogenes (11.64mm). Cymbopogon citratus leaf essential oil (citral) had mild reactivity against the two organisms while Cefuroxime and Meropenem showed severe reactivity against E. coli and E. aerogenes, respectively
