Introduction: The production of extended-spectrum- β lactamases (ESBLs) is an important mechanism for resistance to the third generation cephalosporins. Awareness and the detection of these enzymes are necessary for optimal patient care.Methodology: The total of 96 isolates which were isolated from the different samples tested for ESBL production. This study was conducted in Department of Microbiology, Heritage institute of Medical Sciences.Result: This result of this study revealed those 69.7% organisms which were ESBL producers & 30.2% organisms which were non ESBL producers.Conclusion: It is essential to report ESBL production along with the routine sensitivity reporting, which will help the clinician in prescribing the proper antibiotics
