Kristen H. Hutson and Kaitlynn M. Willis are students in the School of Biological Sciences at Louisiana Tech University.
Gergana G. Nestorova is an Assistant Professor in the School of Biological Sciences at Louisiana Tech University

Hutson, Kristen H.

Nestorova, Gergana G.

Willis, Kaitlynn

English

Louisiana Tech Digital Commons

Louisiana Tech UniversityLouisiana Tech Digital CommonsANS Research Symposium ANS Research Symposium 2018Apr 12th, 8:30 AM - 11:30 AMIdentification of Novel MicroRNAs that RegulateOGG1 Mediated DNA RepairKristen H. HutsonLouisiana Tech UniversityKaitlynn WillisLouisiana Tech UniversityGergana G. NestorovaLouisiana Tech UniversityFollow this and additional works at: https://digitalcommons.latech.edu/ans-research-symposiumThis Event is brought to you for free and open access by the Conferences and Symposia at Louisiana Tech Digital Commons. It has been accepted forinclusion in ANS Research Symposium by an authorized administrator of Louisiana Tech Digital Commons. For more information, please contactdigitalcommons@latech.edu.Recommended CitationHutson, Kristen H.; Willis, Kaitlynn; and Nestorova, Gergana G., "Identification of Novel MicroRNAs that Regulate OGG1 MediatedDNA Repair" (2018). ANS Research Symposium. 29.https://digitalcommons.latech.edu/ans-research-symposium/2018/poster-presentations/29 41   Identification of novel miroRNAs that regulate OGG1 mediated DNA repair Kristen H. Hutson1, Kaitlynn M. Willis1, Gergana G. Nestorova 1  1School of Biological Sciences, Louisiana Tech University 2Assistant Professor, School of Biological Sciences, Louisiana Tech University  Reactive oxygen species induce modifications of the DNA bases that are implicated in cancer development and progression as well as aging and age-related neurological disorders. The base excision repair mechanism had evolved to repair the mutations induced by oxygen radicals. The objective of this study is to identify novel microRNAs that regulate the expression of 8-oxoguanine glycosylase (OGG1), an enzyme that plays an important role in the DNA base excision repair pathway. Altered expression of OGG1 leads to accumulation of modified bases, DNA damage, and increased rate of nucleic acid mutation. To simulate conditions of oxidative stress, human astrocytes were treated for 16 hours with 10µM sodium dichromate. OGG1 mRNA and protein expression levels were assessed via RT-qPCR and protein simple Wes® assay. Comet assay analysis were performed to assess the level of oxidative stress induced DNA damage. RNA extracted from treated and non-treated cells was sequenced using Ion Proton small RNA sequencing platform. OGG1 mRNA and protein expression levels were significantly reduced after treatment with sodium dichromate. Comet assay analysis confirmed high levels of oxidative stress induced DNA damages. MicroRNA sequencing revealed that large number of microRNAs are up regulated following treatment with sodium dichromate. Bioinformatics analysis was implemented to identify potential microRNAs targets that bind to the 3’UTR region of the OGG1 mRNA gene. Those include miR-20b, miR-33, miR-let7, miR- 103, and miR-491. The binding between the potential miroRNA candidates and OGG1 gene will be validated via immunoprecipitation studies.                     

Identification of Novel MicroRNAs that Regulate OGG1 Mediated DNA Repair

https://digitalcommons.latech.edu/cgi/viewcontent.cgi?article=1043&context=ans-research-symposium

Identification of Novel MicroRNAs that Regulate OGG1 Mediated DNA Repair

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