Objective: Inflammation is a protective response to tissue injury caused by physical trauma, harmful chemicals, or microbiological substances.Leukotriene, which originates in the lipoxygenase pathway, is a mediator of inflammation. Averrhoa carambola L. leave contains flavones, includingapigenin, which exhibits potential anti-inflammatory activity through the inhibition of lipoxygenase activity. The present study aimed to examine thelipoxygenase inhibition activity of A. carambola leaf extracts.Methods: A. carambola leaf extracts were obtained using multilevel maceration using the solvents n-hexane, ethyl acetate, and ethanol. Subsequently,total flavonoid contents in the three extracts were determined using the colorimetric method. Apigenin content in the most active extract wasdetermined using high-performance liquid chromatography.Results: The extract with the highest lipoxygenase inhibition activity was the ethyl acetate extract with an IC50 value of 10.17±0.83 μg/mLfollowed by the n-hexane and ethanol extracts with IC50 values of 40.99±0.51 and 35.61±0.66 μg/mL, respectively. Total flavonoid content fromthe n-hexane, ethyl acetate, and ethanol extracts was 3.01, 24.24, and 8.24 mg quercetin equivalent/g extract, respectively. A qualitative test usingthin-layer chromatography showed that all extracts contained apigenin. The most active (ethyl acetate) extract contained 5.39% apigenin with ahigh-performance liquid chromatography method.Conclusion: The ethyl acetate extract of A. carambola leaf is the most active extract to inhibit lipoxygenase activity. Flavonoid content has a strongcorrelation to the inhibitory activity of each extract
