GAS CHROMATOGRAPHY - MASS SPECTROMETRY ANALYSIS, INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY INVESTIGATION, AND ANTIMICROBIAL SCREENING OF CAESALPINIA BONDUCELLA (L.) ROXB SEED KERNEL

Abstract

Objective: The objective of this study was to determine heavy metals using inductively coupled plasma mass spectrometry (ICP-MS) and phytochemical constituents of methanol extract of Caesalpinia bonducella seed kernel using gas chromatography - mass spectrometry (GC-MS). Methods: In GC-MS investigation, 95% methanol extract of C. bonducella seed kernel was performed on JEOL GC MATE ΙΙ, column HP 5 MS, and Quadruple, double-focusing mass analyzer. Determination of heavy metals from C. bonducella powder was performed using Thermo scientific I CAP Q instrument, for plant powder digestion Anton Paar Microwave model: Multiwave 300 was used. Antimicrobial activity was evaluated using microdilutions broth method. Results: GC-MS analysis of methanol extract of C. bonducella seed kernel has shown 11 different phytoconstituents. ICP-MS analysis has shown the concentration of ten heavy metals in C. bonducella seed kernel as follows: Cr 1.5 ppm, Fe 72.72 ppm, Co 0.33 ppm, Ni 0.03 ppm, Cu 10.73 ppm, Zn 18.44 ppm, As 0.03 ppm, Cd 0.02 ppm, and Pb 0.58 ppm. Conclusion: Result showed that the plant is safe to use from toxic heavy metals such as As, Cd, and Pb. It is suitable to check frequently the heavy metal content in the plant used in traditional medicines before given to patients