Objectives: Urinary tract infection (UTIs) caused by Citrobacter species have been described in 5 to 12% of bacterial urine isolates in adults. The genus Citrobacter is a distinct group of aerobic Gram-negative bacilli from the Enterobacteriaceae family. We report here the emergence of Citrobacter as an increasingly common urinary pathogen in patients attending this medical college.Methods: The study was carried out over a period of 2 years from January 2010 to December 2011. Urine specimens from both outpatients and inpatients of our hospital were processed. Urine samples which yielded the growth Citrobacter spp. were included in the study Results: E. coli was the most common organism isolated followed by Klebsiella spp. and Citrobacter spp. The most active antimicrobial agent against Citrobacter isolates was piperacillin-tazobactum (55% sensitive), closely followed by amikacin (47%).Conclusion: The emergence of this usually rare organism as the third most common urinary pathogen, which is resistant to commonly available antibiotics is alarming. Such studies will guide clinicians to choose accurate empirical treatment options and will help to reduce the mortality and morbidity rates from infections. Â