Objective: The objective of this research was to develop the nano-particles of natural colours from Ratanjot root, Seabuckthorn pulp, and Annatto seeds and use them in cosmetic products. Multiple herbs were used to have a good mix of active colour constituents and have a possibility to create a range of shades in the final cosmetic formulation without any side-effects. Further, with nano-sizing of the colour constituents, we aimed to obtain better activity and enhanced performance in the final product.Methods: The extraction of natural colours from herbal source was carried out under controlled conditions of temperature (max. 50 °C) and pressure (50-60 mm of Hg) to minimize the loss of actives during the process. The colour fractions separated by solvent fractionation were nano-sized by ultra-sonication using polyethylene glycol 400 as additive to distribute uniformly in the formulation. Active colour components were analyzed by HPLC and Lovibond Tintometer to maintain the colour strength in cosmetic formulation.Results: The nano-sizing of natural colour (purplish red dye from Ratanjot root, oil of Seabuckthorn pulp) has resulted in giving an excellent gloss, spreadability and prevention of skin damage due to their good anti-oxidant property. The goodness of Seabuckthorn oil has rendered a special effect of rejuvenating the skin cells to retain their elasticity.Conclusion: The promising results of these formulations have encouraged us to go for pilot trials to extend the study on animal and human subjects for the skin-penetration and kinetics of their healing power.Keywords: Bixa orellana, Arnebia nobilis, Hippophae rhamnoides, Herbal lipstick, Herbal lip balm, Body Massage oil, Cosmetics, Carotenoid
