Objective: This report is an attempt to evaluate methanolic extracts of leaves, fruits and bark from Dalbergia sissoo for photoprotective and DNA protective potential in vitro and to get an insight into its phytochemical constituents.Methods: Total methanolic extracts of leaves, fruits and bark from D. sissoo were examined for total phenolics content (TPC), total flavonoids content (TFC) and antioxidant activity. Extracts alone, as well as part of gel formulations, were used to assess their sun protection factor (SPF) by spectrophotometry. Potential of extracts to prevent plasmid DNA damage by hydroxyl radicals generated through Fenton and ultraviolet+hydrogen peroxide (UV+H2O2) system was checked by agarose gel electrophoresis. GC-MS analysis was done to detect the presence of bioactive compounds in active fractions.Results: TPC, TFC, antioxidant activity and SPF values of bark methanolic extract (BME) were found to be highest among the three extracts. Dose-dependent protection of DNA was observed in the extracts. Phytol, lupeol, squalene, palmitic acid and phytosterol etc. were the key constituent of the extracts.Conclusion: As per the available literature, this is the first time exploration of D. sissoo methanolic extract as photoprotective and DNA protective agents. Total extracts or its purified fractions can be exploited as Cosmeceutical ingredient.Keywords: Dalbergia sissoo, Phytochemicals, Antioxidants, SPF, pBluescript, Fenton, H2O2 and U
