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MYC regulates metabolism through vesicular transfer of glycolytic kinases
Authors
S Bibbò
G Cangemi
+15 more
E Capone
VC Carregari
O Chayka
L Chesler
V De Laurenzi
A Eva
MR Larsen
E Makarov
M Morini
G Palmisano
L Pieroni
E Poon
A Sala
G Sala
A Tsakaneli
Publication date
1 December 2021
Publisher
'The Royal Society'
Doi
View
on
PubMed
Abstract
Electronic supplementary material is available online at https://doi.org/10.6084/m9.figshare.c.5713034.Copyright © 2021 The Authors. Amplification of the proto-oncogene MYCN is a key molecular aberration in high-risk neuroblastoma and predictive of poor outcome in this childhood malignancy. We investigated the role of MYCN in regulating the protein cargo of extracellular vesicles (EVs) secreted by tumour cells that can be internalized by recipient cells with functional consequences. Using a switchable MYCN system coupled to mass spectrometry analysis, we found that MYCN regulates distinct sets of proteins in the EVs secreted by neuroblastoma cells. EVs produced by MYCN-expressing cells or isolated from neuroblastoma patients induced the Warburg effect, proliferation and c-MYC expression in target cells. Mechanistically, we linked the cancer-promoting activity of EVs to the glycolytic kinase pyruvate kinase M2 (PKM2) that was enriched in EVs secreted by MYC-expressing neuroblastoma cells. Importantly, the glycolytic enzymes PKM2 and hexokinase II were detected in the EVs circulating in the bloodstream of neuroblastoma patients, but not in those of non-cancer children. We conclude that MYC-activated cancers might spread oncogenic signals to remote body locations through EVs.Neuroblastoma UK to AS and FAPESP SPRINT Award (50356-4); FAPESP (2014/06863-3, 2018/18257-1, 2018/15549-1, 16/50356-4); CNPq “bolsa de produtividade”; Ricerca Finalizzata GR11-172.https://doi.org/10.6084/m9.figshare.c.571303
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Last time updated on 11/01/2022