Introduction: Health workers have important roles to play in reducing mother-to-childtransmission of HIV. This study assessed health workers' knowledge of the nationalguidelines on preventing mother-to-child transmission of HIV in Benin City, Edo State,NigeriaMethodology: The study design was a descriptive cross-sectional survey. The studypopulation comprised health workers providing services for preventing mother-to-childtransmission of HIV in Benin City, Edo State, Nigeria. Data was collected through selfadministeredquestionnaires.Results: Two hundred and seventy health workers participated in the study comprisingdoctors, nurses, pharmacists, laboratory scientists and counsellors. All the respondentshad heard of preventing mother-to-child transmission of HIV. Ninety-two (34.1%) hadreceived training on the national guidelines on preventing mother-to-child transmissionof HIV. The proportion of health workers with poor, fair, and good knowledge of thenational guidelines on PMTCT was 8.5%, 30.4% and 61.1% respectively. Knowledge ofthe national guidelines was significantly associated with the health workers' occupation,previous training on PMTCT and having a copy of the national guidelines.Conclusion: This study highlighted important gaps in health workers knowledge ofPMTCT. Training and retraining of health workers remains crucial to achieving the goalof reducing mother-to-child transmission of HIV. The study findings may guide thecontent of future training of health workers on PMTCT to ensure relevance andadequacy
