Conservation planning in the Cape Floristic Region, a recognized world plant diversity hotspot, required systematic (i.e. presence/absence) information on the estimated distributions of the medium- to large-sized mammals. A pragmatic approach for obtaining distribution estimates, for the period prior to arrival of European settlers, was employed. Distribution estimates were based on a combination of a literature survey (with emphasis on early texts) and the ecological requirements of species, and were mapped within each of 102 Broad Habitat Units delineated according to key biophysical parameters. The estimated distributions of 42 species are provided in the form of maps; these are accompanied by brief notes on historical and current occurrences. The distributions, which can be used to guide conservation decisions, should be considered as testable hypotheses.Key words: Cape Floristic Region, distributions, mammals, habitats