Hypoxia is a major factor that affects the subsistence and development of multicellular organisms. Tibet chicken, as a unique native chicken breed in altiplano, shows genetic adaptation to hypoxia comparing with the breeds at the low altitude. In the present study, to explore effects of hypoxia on chicken fetal livers, eggs of Tibet chicken and Shouguang chicken were collected and the samples from each breed were divided into two groups, incubated in hypoxia and in normoxia respectively. The blood of embryos on the 16th day of incubation was collected and the serum chemistry parameters indicating liver metabolism were determined, which included glutamic-pyruvic transaminase (GPT), aspartate aminotransferase (GOT), total bilirubin (TB), direct bilirubin (DB), total bile acid (TBA), gamma glutamyltransferase (GGT), alkaline phosphatease (ALP), lactate dehydrogenase (LDH), creatine kinase (CK), glucose and creatinine. The results show that biochemical indices varied significantly between hypoxia and normoxia except for GPT and glucose. Moreover, the concentration of ALP and LDH showed significant differences between the breeds and the incubations. The results suggest that the livers of both Shouguang chicken and Tibet chicken suffered damages in hypoxia, but the former was more serious. The results of this study support the opinion that Tibet chicken had better genetic adaptability on hypoxia, and made a good basis for further study of the genetic mechanism of adaptation to hypoxia.Key words: Hypoxia adaptation, liver metabolism, serum chemistry, Tibet chicken, chicken embryo