Seven microsatellite markers OarAE101, BM1329, OarHH55, BM143, BMS2508, OarAE129 and OarFCB11 closely associated with high reproduction trait in sheep were analyzed for polymorphisms in Xinong Saanen dairy goat. The results indicated that there were high genetic polymorphisms at six microsatellite loci. The number of effective alleles (Ne), polymorphism information content (PIC) and average heterozygosity (He) were the highest at OarFCB11 and the lowest at OarAE129 in Xinong Saanen dairy goat. The analysis of the effect of the six polymorphisms microsatellite loci on the litter size of Xinong Saanen dairy goat indicated that these polymorphisms microsatellite loci had positive effect on the litter size.Key words: Microsatellite markers, Xinong Saanen dairy goat, genetic polymorphism, litter size