As in many lichen-forming fungi, species of Aspicilia genus are widely distributed all over the world, but no reports exist about their phylogenetic relations based on molecular biological methods. In thecurrent study the phylogenetic relations of some Aspicilia species mainly manna group of lichens were investigated. The ITS rDNA sequence information of 12 samples from six species were generated. The samples examined were collected from different provinces of Anatolia and all the sequences were aligned with the other allied groups; Pertusaria sp., Thamnolia sp., Dibaeis sp., Diploschistes sp.,Ochrolechia sp. and Lecanora sp. sequence data obtained from GenBank. The phylogenetic tree obtained by minimum evolution analysis displayed two major branches. One of the branches with itssix members (Aspicilia contorta subsp. contorta, A. contorta subsp. hoffmanniana, Aspicilia hispida, Aspicilia fruticulosa, Aspicilia desertorum Aspicilia calcarea) composed solely of Aspicilia samplesfrom Anatolia. Three of the manna groups of lichens were placed in this branch of the tree. The other related taxa from Ostropomycetidae and Lecanoromycetidae took place in the other branch of the treewith Aspicilia samples from Anatolia. Results displayed that manna group of lichens, although do not represent taxonomical unit tend to form a group especially apperent by the Neigbour Joining analysiswith Anatolian samples. Also the sequence information from Anatolian samples displayed that Aspicilia genus is phylogenetically closer to the orders and families from Ostropomycetidae subclass rather than Lecanoromycetidae which support the placement of Hymeneliaceae within Ostropomycetidae
