Laboratory analysis of the water extract of Tithonia diversifolia shoot and root on infra-red spectrometer to determine the phytotoxic potential and greenhouse experiments to evaluate its phytotoxic effect and growth promoting attribute on cowpea (Vigna unguiculata L.) were carried out. T. diversifolia contained some compounds having functional groups such as alcohols, olefins, six-membered ring lactams, ester, aromatic compound which can also be found in sesquiterpene lactones, an allelochemical implicated in phytotoxicity. The extract promoted seedling and plant growth. Bacillus and Streptococcus spp. earlier isolated from the root rhizosphere of T. diversifolia caused some changes in the spectra of the component compounds in the extract. The plant extract promoted growth of cowpea.African Journal of Biotechnology Vol. 4 (4), pp. 355-360, 200