Gentiana L. species are distributed in the North hemisphere, in Europe, Asia, North America, and in South America they occur in the Andes. In Europe, 29 species occur and are classifed into six sections, determined by their morphological and physiological characteristics [1]. Some of them are distributed in the mountainous part of Romania [2]. The paper presents the results of a survey made in the Postavaru Mountains, in Romanian country, on mapping and monitoring of certain populations of Gentiana lutea L., an endangered species. The aim of these researches was to create a germoplasm fund to be used further as initial material for breeding works, in order to create new genotypes, able to be cultivated on large scale. We realized to identified some families from Gentiana lutea L. species who present different fenotipycal caracters. RAPD analysis can use for establish the degree of their genetic variability at the DNA level. Since so many species and varieties exist, development of molecular markers would be important for quality assessment in the medicinal industry. Recently, restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) analyses have been carried out on Gentiana genus [1]. RADP can be employed as a means of differentiating or characterizing subspecies, varieties or ecotypes. Our work want to present the genetic fingerprints for ten families by Gentiana lutea L. A dendrogram (fig. 1) of the relative relation between these families has been drawn based on the DNA polymorphism as revealed by the RAPD markers. The molecular data was compared with, and corroborated to the phenotypic data in order to achieve a better picture of relation and variation between these ten lines
