A neuroendocrine reflex attributed to the mechanical stimulation of sensory receptors during copulation induces ovulation in cats. This stimulation can be simulated with a vaginal swab, triggering pseudopregnancy. The efficiency of ovulation induction with swab was evaluated in order to establish a natural contraceptive treatment for domestic cats as well as to analyze the effect on uterus of the repeated use of this technique. At the first phase of the study, 12 animals were evaluated in three consecutive estrous cycles. In the first cycle (T1), vaginal swab stimulation was applied. In the second cycle (T2), a vasectomized male was used. In the third cycle (T3), ovulation was accompanied unstimulated (control). At the second phase of the study, 13 female cats were induced to successive pseudopregnancy states to check the effects on the uterus. Confirmation of ovulation in the two phases of the study was carried out by measuring progesterone serum levels. The vaginal swab stimulation showed a similar response to that obtained by natural mating (P>0.05). Some cats showed mild uterine changes without pathological relevance. Thus, ovulation induction with swab is secure and without significant side effects.Keywords: contraceptive; feline; oestrus; ovulation