A. A. Kostareva

A. A. Sergushichev

A. M. Kiselev

N. K. Tokarevich

O. A. Freylikhman

S. V. Kazakov

Yu. A. Panferova

Russian Journal of Infection and Immunity

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5452018, vol. 8, no. 4 4. Zoonotic and parasitic infectionsticks with Borrelia burgdorferi sensu lato (B. burgdorferi s.l.) and Borrelia miyamotoi in the Khabarovsk region during 2017–2018.A total number of 1238 ixodic ticks were tested on the presence of the B. burgdorferi s.l. and 710 ticks were tested on the presence of B. miyamotoi DNA via Real-time PCR. Identification of the nucleic acids of the pathogens was performed using PCR kits “RealBest DNA B. miyamo-toi”, “RealBest DNA B. burgdorferi s.l.” (“Vector-Best”, Novosibirsk) according to the manufacturer’s instructions.The infestation rate of Ixodes persulcatus ticks (31.4±1.74%) with B. burgdorferi s.l. was statistically higher compared to Dermacentor silvarum (12.8±4.87%, p < 0.05) and Haemaphysalis spp. (16.6±2.86%, p < 0.05). No signif-icant difference between infestation rates of different spe-cies of ticks with B. miyamotoi was found. During the start of the epidemic season was registered an elevation of vec-tors infestation rate with B. burgdorferi s.l. with a peak in July (33.1±4.33%, p < 0.05) followed by a consequent decline down to 11.8±5.53% (p < 0.05) in September. A decline in infestation rate of B. miyamotoi vectors from 10.4±2.63 to 5.8±1.46% (p < 0.05) was registered. From July to September the DNA of B. miyamotoi was not found. It is of importance that 14 ticks had a coinfection with B. burgdorferi and B. miyamotoi in 2017–2018. The Ct DNA value of B. burgdorferi s.l. was higher in most of the cases compared with Ct DNA value of B. miyamotoi.The infestation rate of Ixodic ticks with B. miyamo-toi was significantly lower compared to B. burgdorferi s.l. in Khabarovsk Region. The obtained results imply that during the start of the epidemic season (April-May) the risk of exposure of the population to B. miyamotoi is higher compared to summer-autumn period. Thus, it is important to study the competition between the pathogens in ticks and its value on the manifestation of the diseases in humans.4.9 doi: 10.15789/2220-7619-2018-4-4.9PATHOGENS, PESTICIDE RESISTANCE AND GENETIC DIVERSITY OF HUMAN HEAD LICEM.E. Eremeeva1, M. Anderson1, T. Ogunleke1, S. Warang1, D. Capps1, S. Zohdy2, L.A. Durden31Jiann-Ping Hsu College of Public Health, Georgia Southern University, Statesboro, USA; 2School of Forestry & Wildlife Sciences, Auburn University, Auburn, USA; 3College of Science and Mathematics, Georgia Southern University, Statesboro, USAPediculosis capitis or head louse infestation is the most prevalent parasitic infestation of humans. It is commonly perceived as an age-dependent rite of pas-sage, and as an embarrassing social nuisance; however, head louse outbreaks do not raise any substantial pub-lic health concerns due to their assumed low capacity for transmitting the louse-borne pathogens associat-ed with Pediculus humanus humanus, the human body louse. The purpose of this study was to screen head lice, Pediculus humanus capitis from Georgia, USA and Madagascar for Bartonella quintana and Acinetobacter sp. to determine the risk of exposure of rural populations with different levels of economic development to these pathogens. Other aims were to examine these lice for the occurrence of genetic markers for permethrin resist-ance using restriction fragment length polymorphism (RFLP/PCR) analysis and to evaluate the genetic struc-ture of these head louse populations using microsatellite typing. The kdr permethrin resistance biomarker for the T917I mutation was detected by RFLP/PCR in 99.9 and 70% of lice from Georgia and Madagascar, respectively. Bartonella DNA was detected at similar levels in both set of samples (10.3 and 12.6%), while Acinetobacter sp. DNA was detected more frequently in Georgia lice (80.8%) than in Malagasy lice (42.1%). Microsatellite typing based on 3 sites revealed significant genetic he-tero geneity among the lice tested, although head lice from Georgia were separated in 2 closely related clusters, while Malagasy lice exhibited more genetic diversity us-ing Principal Component Analysis and Bayesian cluster-ing. The results provide the first information regarding these combined characteristics of head louse infestations at these locations and can be used as a baseline for tem-poral surveillance of changes in circulating head louse populations, for monitoring louse susceptibility to per-methrin-based pediculicides, and to track potential ex-posures and outbreaks due to louse-borne pathogens.4.10 doi: 10.15789/2220-7619-2018-4-4.10WHOLE GENOME-BASED CHARACTERIZATION OF COXIELLA BURNETII STRAINS ISOLATED IN RUSSIAN FEDERATIONO.A. Freylikhman1,2, A.M. Kiselev2, A.А. Sergushichev3, S.V. Kazakov3, Yu.A. Panferova1, A.A. Kostareva2,3, N.K. Tokarevich11St. Petersburg Pasteur Institute, St. Petersburg, Russia; 2Federal Almazov North-West Medical Research Centre, St. Petersburg, Russia; 3ITMO University, St. Petersburg, RussiaInformation on the whole genome structure of the Coxiella burnetii strains circulating in Russian Federation (RF) is currently unavailable. The identification and study of differential-significant genetic markers of C. bur-netii that are important from the epidemiological point of view remains a priority, including determining the hos-tal speci ficity.The aim was whole genome-based characterization of C. burnetii strains isolated from different hosts.We performed whole genome sequencing of four C. bur-netii strains isolated from two host types (the human and the arthropods) in RF using the MiSeq technology (Illumina, USA). Genome assembling and alignement using Dugway 5J108-111 as the reference was performed with SPADes 3.9.0 genome assembler. Comparative ana-lysis of the whole genomes of Russian strains was carried out between the investigated genomes and the whole ge-nomes sequences of C. burnetii available in the NCBI da-tabase.It was confirmed the conception of closed pangenome of species C. burnetii characterized by a low intraspecies ge-netic diversity, including the population circulating in the territory of RF. However, in Russian strains of C. burnetii unlike foreign strains the analysis of the variable part of the genome and the composition of unique genes revealed de-letions of a part of them, which allows us to speak about their unique genomotype. Analysis showed pronounced clustering within a group of Russian strains by host type, the differences between genomes within clusters were mi-nor. Comparing the number of deleted genome fragments it was found that surprisingly strains from arthropods had a significantly greater genome reduction compared with strains from human. These data are in contrast with the conclusions of a number of authors that the genomic reduction of C. burnetii strains isolated from arthropods is limited.Thus, this corpus of data allow us to characterize the ge-nomes of C. burnetii strains isolated in the territory of RF and to make assumptions about the hostal specificity of this pathogen, prompting further studies of its mechanisms.

WHOLE GENOME-BASED CHARACTERIZATION OF COXIELLA BURNETII STRAINS ISOLATED IN RUSSIAN FEDERATION

https://doaj.org/article/47261f550a9d46eeb47873dd29b5e455

WHOLE GENOME-BASED CHARACTERIZATION OF COXIELLA BURNETII STRAINS ISOLATED IN RUSSIAN FEDERATION

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