A. A. Serov

A. I. Ruleva

L. S. Fedorova

N. V. Shestopalov

T. V. Gololobova

Russian Journal of Infection and Immunity

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6092018, vol. 8, no. 4 9. Antibiotic resistance of microorganismsthan standard strains S. aureus 25923 АТСС). Density of C. albicans 609 biofilm exceed the density of the stan dard strain C. albicans CCM 885 biofilm at 14.5%. However, dual bacteria-fungal biofilm S. aureus U14 + C. albicans 609 ex-ceed density of biofilm S. aureus 25923 АТСС + C. albicans CCM 885 more than twice (208%). Dual-species biofilm isolated from throat healthy media, S. aureus 609 + C. albi-cans 609, did not differ in density from dual-species biofilm of reference cultures.Thus, when coupled with cultivation yeast C. albicans in vitro aggressiveness of S. aureus clinical strain was in-creased. Carrier state yeast fungi, therefore, could serve as a risk factor for developing a more dense staphylococcal biofilm on the mucous membranes and worsen the course of the disease.9.23 doi: 10.15789/2220-7619-2018-4-9.23MICROBIOLOGICAL MONITORING OF THE RESISTANCE OF HOSPITAL BACTERIAL FLORA WITHIN THE SYSTEM OF PREVENTION OF HEALTHCARE-ASSOCIATED INFECTIONSN.V. Shestopalov1,2, T.V. Gololobova1,2, L.S. Fedorova1, A.A. Serov1, A.I. Ruleva31Research Institute of Disinfectology, Moscow, Russia; 2FSBEI FPE RMACPE MOH Russia, Moscow, Russia; 3Treatment and Rehabilitation Center, Moscow, RussiaEffective implementation of disinfection activities in healthcare organizations (HCO) plays a great and, sometimes, even a critical role in the prevention of health-care-associated infections (HAI). Formation and spread of microorganisms which are resistant to used chemical disinfectants in HCO substantially reduce the effect of dis-infection measures. This, in turn, is the common reason of the high level of HAI incidence. The problem is aggra-vated by the development of antibiotic resistance among the strains which are resistant to DA (cross-resistance).According to the requirements of Sanitary Regulations and Norms 2.1.3.2630-10, it is necessary to conduct moni-toring of hospital bacterial flora sensitivity to disinfection agents (DA).The assessment of sensitivity of microorganisms isolat-ed from the objects of intrahospital environment of inten-sive care, intensive therapy and surgical units — 20 strains (29%), and from the pathological loci of in-patients — 50 strains (71%) resistant to various groups of antibiotics (K. pneumonia, A. baumannii, P. aeruginosa, P. mirabilis, S. maltophilia) — was carried out.Testing was conducted according to the method described in Methodical Guidelines 3.5.1.3438-17 “As-sessment of sensitivity to disinfection agents demonstrated by microorganisms circulating in healthcare organiza-tions”. To compare the resistance of hospital microorga-nisms with that of the microorganisms from the collection which demonstrate standard resistance to DA a collection strain P. aeruginosa ATCC 27853 was used.It was determined that 11 strains were resistant to cati-onic surface-active agents, 10 strains — to active oxygen, 6 strains — to the combination of quaternary ammonium compounds and active oxygen. 27 (38.7%) out of 70 strains of microorganisms isolated from external environment ob-jects and patients were resistant to used DA.The identity of resistant strains isolated from external environment and patients serves as evidence of cross-con-tamination and leads to the spread of resistant strains among patients which, in turn, determines the need to im-prove the organization of preventive activities.9.24 doi: 10.15789/2220-7619-2018-4-9.24GENETIC VARIANTS OF RESISTANCE DETERMINANT TO SILVER IN EPIDEMIC STRAINS OF ACINETOBACTER BAUMANNIIA.P. Solomennyi1, N.A. Zubareva21Institute of Ecology and Genetics of Microorganisms, Federal Research Centre of Ural Branch of RAS, Perm, Russia; 2E.A. Wagner State Medical University, Perm, RussiaSilver-containing dressings are widely used in burns and leg ulcers for prevention and treatment infection. Heavy metal pump CzcA of RND transporters family pre-dicted roles in the efflux both toxic cations including silver and some antibiotics from bacterial cells. Molecular epi-demiology and surveillance of outbreaks for last decades indicate that the most resistant Acinetobacter baumannii belong to two globally disseminated clonal lineages, GC1 and GC2. Strains of GC2 are epidemic as for Far East and Indochina. Our study was designed in order to clari-fy an evolution of silver resistance determinant in clinical A. baumannii.Multiple-resistant strains from different geographic locations were selected. List of strains included A. bau-mannii AYE (GC1, epidemic in France for past years) and strains of GC2: ACICU isolated in an outbreak (Italy), sturdy-bio film forming 1656-2 (South Korea), LY9 and BJ5 recovered in Southern and Northern China hospitals, consequently and strain of sequence-type ST2 (Institut Pasteur typing scheme) endemic in Perm in 2010–2011. Sequences of czcA were retrieved from GenBank database for in silico comparative analysis using BLAST.Surprisingly, czcA gene on chromosomes of A. bau-mannii GC1 and GC2 spread over the world is presented as two prevailing alleles only (allele 1 in AYE, ACICU, 1656-2 and allele 2 in LY9, BJ5 and Perm). Even di- and multinucle-otide variants on positions 213–215 ACC or TTT, 897–898 TG or AC, 945–948 CCGT or TAAA and 951–952 AG or TA have been related to distinct allele (nuc leotide numbers from start codon ATG).The important mechanism of A. baumannii survival under silver presence is expressed in an extreme decrease in the genetic heterogeneity of encoding sequence.This study was supported by the Federal research programme under state registration number 01201353247.9.25 doi: 10.15789/2220-7619-2018-4-9.25COMMENSAL STRAINS OF ESCHERICHIA COLI AND BETA-LACTAM RESISTANCEL.V. Suzhaeva, S.A. Egorova, L.A. KaftyrevaSt. Petersburg Pasteur Institute, St. Petersburg, RussiaAccording to the World Health Organization, the an-timicrobial resistance (AMR) remains a huge worldwide problem of our time which we yet have to overcome. One of the ways to contain the antibiotic resistance is to monitor а circulation of resistant strains of microorganisms, as well as genes that determine the AMR. Studies in recent years have shown a high level of resistance in Klebsiella pneu-moniae and Escherichia coli, the causative agents of no-socomial and community-acquired infections. However, resistant strains also may be a part of gastrointestinal mi-crobiota in healthy individuals.Antimicrobial susceptibility of 511 commensal E. coli strains isolated from faeces of children in age groups from 1 month to 17 years old living in St. Petersburg to 9 groups of antibiotics have been studied by disco-diffusion me thod. The resistance mechanisms among strains insusceptible 

MICROBIOLOGICAL MONITORING OF THE RESISTANCE OF HOSPITAL BACTERIAL FLORA WITHIN THE SYSTEM OF PREVENTION OF HEALTHCARE-ASSOCIATED INFECTIONS

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