Modeling of 5-fluorouracil infulence on activity of its’ metabolic enzymes

Abstract

Stashkevych M. Modeling of 5-fluorouracil infulence on activity of its’ metabolic enzymes. Journal of Education, Health and Sport. 2015;5(11):62-67. ISSN 2391-8306. DOIhttp://dx.doi.org/10.5281/zenodo.33358 http://ojs.ukw.edu.pl/index.php/johs/article/view/2015%3B5%2811%29%3A62-67 https://pbn.nauka.gov.pl/works/666610 Formerly Journal of Health Sciences. ISSN 1429-9623 / 2300-665X. Archives 2011–2014http://journal.rsw.edu.pl/index.php/JHS/issue/archive   Deklaracja. Specyfika i zawartość merytoryczna czasopisma nie ulega zmianie. Zgodnie z informacją MNiSW z dnia 2 czerwca 2014 r., że w roku 2014 nie będzie przeprowadzana ocena czasopism naukowych; czasopismo o zmienionym tytule otrzymuje tyle samo punktów co na wykazie czasopism naukowych z dnia 31 grudnia 2014 r. The journal has had 5 points in Ministry of Science and Higher Education of Poland parametric evaluation. Part B item 1089. (31.12.2014). © The Author (s) 2015; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland and Radom University in Radom, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 05.09.2015. Revised 05.10.2015. Accepted: 30.10.2015.   MODELING OF 5-FLUOROURACIL INFULENCE ON ACTIVITY OF ITS’ METABOLIC ENZYMES   M. Stashkevych   O. O. Bogomolets National Medical University   Abstract This article presents the results of model experiment which involved intraperitoneal administration of 5-fluorouracil (5-FU) to rats with further determination of the activity of its’ key metabolic enzymes: dehydropyrimidine dehydrogenase (DPD) and thymidine phosphorylase (TP), in various time intervals after 5-FU injection. These results were compared to ones from the control group of animals which did not receive 5-FU. It was revealed that 5-FU has the suppressive influence on TP up to 12 hours after injection and does not affect activity of DPD. As these enzymes are responsible for 5-FU activation (TP) and degradation (DPD) the observed influence of the drug is desired effect associated with lesser toxic impact on normal cells.   Keywords: 5-fluorouracil, dehydropyrimidine dehydrogenase, thymidine phosphorylase, rat liver