This study was conducted in order to identity Qualitative factors affecting the production and distribution of information and knowledge in science and technology parks of Iran. The research was Applied Research in which, qualitative method was carried out. The population of the study was included of 10 managers of Knowledge-based Companies. The data was collected from the population using semi-structured and in-depth interviews. For data analysis, content analysis was used. Results of the qualitative factors affecting the production and distribution of information and knowledge in science and technology parks of Iran, led to extraction of 39 components which were classified in four categories: I) Foreign and domestic policy, II) Financial and economic support, III) Infrastructure barriers and IV) Cultural barriers. Results howed that overcoming the political, financial and economic, infrastructural and cultural barriers has undeniable impact on production and distribution of information and knowledge