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Engineering new bone tissue in vitro on highly porous poly(Α-hydroxyl acids)/hydroxyapatite composite scaffolds
Authors
Seung-Hak Baek
Jin-Young Choi
+9 more
Chong-Pyoung Chung
Soo-Boo Han
Seong-Joo Heo
Sukyoung Kim
Perry R. Klokkevold
Yong-Moo Lee
Yun-Tak Lim
In-Chul Rhyu
Yang-Jo Seol
Publication date
1 January 2000
Publisher
'Wiley'
Doi
Abstract
Engineering new bone tissue with cells and a synthetic extracellular matrix (scaffolding) represents a new approach for the regeneration of mineralized tissues compared with the transplantation of bone (autografts or allografts). In the present work, highly porous poly( L -lactic acid) (PLLA) and PLLA/hydroxyapatite (HAP) composite scaffolds were prepared with a thermally induced phase separation technique. The scaffolds were seeded with osteoblastic cells and cultured in vitro . In the pure PLLA scaffolds, the osteoblasts attached primarily on the outer surface of the polymer. In contrast, the osteoblasts penetrated deep into the PLLA/HAP scaffolds and were uniformly distributed. The osteoblast survival percentage in the PLLA/HAP scaffolds was superior to that in the PLLA scaffolds. The osteoblasts proliferated in both types of the scaffolds, but the cell number was always higher in the PLLA/HAP composite scaffolds during 6 weeks of in vitro cultivation. Bone-specific markers (mRNAs encoding bone sialoprotein and osteocalcin) were expressed more abundantly in the PLLA/HAP composite scaffolds than in the PLLA scaffolds. The new tissue increased continuously in the PLLA/HAP composite scaffolds, whereas new tissue formed only near the surface of pure PLLA scaffolds. These results demonstrate that HAP imparts osteoconductivity and the highly porous PLLA/HAP composite scaffolds are superior to pure PLLA scaffolds for bone tissue engineering. © 2000 John Wiley & Sons, Inc. J Biomed Mater Res 54: 284–293, 2001Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/34419/1/16_ftp.pd
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