Degradable and injectable hydrogels may be ideal for bone-tissue engineering, especially in the craniofacial region because of the ease of access for injection. Alginate hydrogels potentially could be used as injectable cell delivery vehicles, but they exhibit a limited range of mechanical properties and uncontrollable disintegration time. Therefore we synthesized new hydrogels, composed of poly(aldehyde guluronate) (PAG) and adipic acid dihydrazide, that have a wide range of mechanical stiffness and controllable degradation rate. MC3T3-E1 cells adhered and multiplied on PAG hydrogels  in vitro . When primary rat calvarial osteoblasts were mixed with PAG hydrogels and subcutaneously injected into the backs of mice, mineralized bone tissues were formed 9 weeks following implantation. These hydrogels may find wide utility as an injectable delivery system for bone precursor cells as well as for other applications in tissue engineering. © 2001 John Wiley & Sons, Inc. J Biomed Mater Res 56: 228–233, 2001Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/34420/1/1089_ftp.pd

Araújo

Baker

Geniès

Leite

MacDiarmird

Nadruz

Parente

Tang

Journal of Biomedical Materials Research

ideal for bone-tissue engineering, especially in the craniofa-cial region because of the ease of access for injection. Algi-nate hydrogels potentially could be used as injectable cell delivery vehicles, but they exhibit a limited range of me-chanical properties and uncontrollable disintegration time. Therefore we synthesized new hydrogels, composed of poly(aldehyde guluronate) (PAG) and adipic acid dihydra-zide, that have a wide range of mechanical stiffness and controllable degradation rate. MC3T3-E1 cells adhered and multiplied on PAG hydrogels in vitro. When primary rat calvarial osteoblasts were mixed with PAG hydrogels and subcutaneously injected into the backs of mice, mineralized bone tissues were formed 9 weeks following implantation. These hydrogels may find wide utility as an injectable de-livery system for bone precursor cells as well as for othe

Kuen Yong Lee

Eben Alsberg

David J. Mooney

CiteSeerX

Degradable and injectable poly(aldehyde guluronate) hydrogels for bone tissue engineering

Crossref

Polyaniline-dacron composite as solid phase in enzyme linked immunosorbent assay forYersinia pestis antibody detection

A new biomimetic method for coating metal implants enables the fast formation of dense and homogeneous calcium phosphate coatings. Titanium alloy (Ti6Al4V) disks were coated with a thin, carbonated, amorphous calcium phosphate (ACP) by immersion in a saturated solution of calcium, phosphate, magnesium, and carbonate. The ACP-coated disks then were processed further by incubation in calcium phosphate solutions to produce either crystalline carbonated apatite (CA) or octacalcium phosphate (OCP). The resorption behavior of these three biomimetic coatings was studied using osteoclast-enriched mouse bone-marrow cell cultures for 7 days. Cell-mediated degradation was observed for both carbonated apatite and octacalcium phosphate coatings. Numerous resorption lacunae characteristic of osteoclastic resorption were found on carbonated apatite after cell culture. The results showed that carbonated apatite coatings are resorbed by osteoclasts in a manner consistent with normal osteoclastic resorption. Osteoclasts also degraded the octacalcium phosphate coatings but not by classical pit formation

Leeuwenburgh, S.C.G.

Layrolle, P.

Barrere, F.

Bruijn, J.G.M. de

Schoonman, J.

Blitterswijk, C.A. van

Groot, K. de

NARCIS 

Osteoclastic resorption of biomimetic calcium phosphate coatings in vitro.

We used a polystyrene substratum to study the response of migrating epithelium to 1- or 5-microm depth microgrooves with groove/ridge widths of 1, 2, 5, or 10 microm. The migration of a tissue sheet was enhanced along the microgrooves, while migration across the microgrooves was inhibited. Changing the depth of the microgrooves had a greater effect on migration than alteration of the groove/ridge width. The migration of epithelial cells from a confluent monolayer culture followed a similar pattern to that of intact epithelial tissue. Cellular extensions generally followed the microgroove direction by tracking along the top of the ridges or following the ridge walls, as revealed by scanning electron microscopy. Actin filaments within the basal cell layer of the tissue were aligned with the microgrooves, unlike filaments in the superficial layers that did not appear to be affected by the presence of underlying microgrooves. The basal cell layer of the tissue conformed to the contours of the microgroove following migration. However, the ultrastructure of the tissue above the ridges resembled that of tissue on a flat surface. We concluded that surface microgrooves have the potential to direct the migration of immediately adjacent epithelial tissue, the effect of which is to guide epithelial tissue on the surface of implanted biomaterials

Dalton, B.A.

Walboomers, X.F.

Dziegielewski, M.

Evans, M.D.

Taylor, S.

Jansen, J.A.

Steele, J.G.

Modulation of epithelial tissue and cell migration by microgrooves.

Item does not contain fulltextA new biomimetic method for coating metal implants enables the fast formation of dense and homogeneous calcium phosphate coatings. Titanium alloy (Ti6Al4V) disks were coated with a thin, carbonated, amorphous calcium phosphate (ACP) by immersion in a saturated solution of calcium, phosphate, magnesium, and carbonate. The ACP-coated disks then were processed further by incubation in calcium phosphate solutions to produce either crystalline carbonated apatite (CA) or octacalcium phosphate (OCP). The resorption behavior of these three biomimetic coatings was studied using osteoclast-enriched mouse bone-marrow cell cultures for 7 days. Cell-mediated degradation was observed for both carbonated apatite and octacalcium phosphate coatings. Numerous resorption lacunae characteristic of osteoclastic resorption were found on carbonated apatite after cell culture. The results showed that carbonated apatite coatings are resorbed by osteoclasts in a manner consistent with normal osteoclastic resorption. Osteoclasts also degraded the octacalcium phosphate coatings but not by classical pit formation

Radboud Repository

The interface between micromachined neural microelectrodes and neural tissue plays an important role in chronic  in vivo  recording. Electrochemical polymerization was used to optimize the surface of the metal electrode sites. Electrically conductive polymers (polypyrrole) combined with biomolecules having cell adhesion functionality were deposited with great precision onto microelectrode sites of neural probes. The biomolecules used were a silk-like polymer having fibronectin fragments (SLPF) and nonapeptide CDPGYIGSR. The existence of protein polymers and peptides in the coatings was confirmed by reflective microfocusing Fourier transform infrared spectroscopy (FTIR). The morphology of the coating was rough and fuzzy, providing a high density of bioactive sites for interaction with neural cells. This high interfacial area also helped to lower the impedance of the electrode site and, consequently, to improve the signal transport. Impedance spectroscopy showed a lowered magnitude and phase of impedance around the biologically relevant frequency of 1 kHz. Cyclic voltammetry demonstrated the intrinsic redox reaction of the doped polypyrrole and the increased charge capacity of the coated electrodes. Rat glial cells and human neuroblastoma cells were seeded and cultured on neural probes with coated and uncoated electrodes. Glial cells appeared to attach better to polypyrrole/SLPF-coated electrodes than to uncoated gold electrodes. Neuroblastoma cells grew preferentially on and around the polypyrrole/CDPGYIGSR-coated electrode sites while the polypyrrole/CH 3 COO − -coated sites on the same probe did not show a preferential attraction to the cells. These results indicate that we can adjust the chemical composition, morphology, electronic transport, and bioactivity of polymer coatings on electrode surfaces on a multichannel micromachined neural probe by controlling electrochemical deposition conditions. © 2001 John Wiley & Sons, Inc. J Biomed Mater Res 56: 261–272, 2001Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/34421/1/1094_ftp.pd

Cui, Xinyan

Lee, Valerie A.

Raphael, Yehoash

Wiler, James A.

Hetke, Jamille F.

Anderson, David J.

Martin, David C.

Deep Blue Documents at the University of Michigan

Surface modification of neural recording electrodes with conducting polymer/biomolecule blends

Lee, Kuen-Yong

Alsberg, Eben

Mooney, David J.

https://deepblue.lib.umich.edu/bitstream/handle/2027.42/34420/1089_ftp.pdf?sequence%3D1

Degradable and injectable poly(aldehyde guluronate) hydrogels for bone tissue engineering

Abstract

Similar works

Full text

Available Versions

CiteSeerX

Crossref

NARCIS

NARCIS

Crossref

Radboud Repository

Deep Blue Documents at the University of Michigan

Deep Blue Documents at the University of Michigan