Pesticide toxicity causes oxidative damage such as DNA damage, enhanced lipid peroxidation, the oxidation of
protein sulfydryl groups and enzyme inactivation in the metabolism. In this study, we investigated the in vitro effects on
glucose 6-phosphate dehydrogenase (E.C.1.1.49; G6PD) from Capoeta capoeta umbla kidney of imidacloprid and lambda-cyhalothrin.
For this purpose, the enzymewas purified from kidney of C. c. umbla with a specific activity of 11.26 EU mg-1
proteins and 22.7% yield using hemolysate preparation, ammonium sulfate precipitation and 2',5'-ADP Sepharose 4B
affinity gel chromatography methods. In order to control the enzyme purification sodium dodecyl sulfate polyacrylamide gel
electrophoresis (SDS-PAGE) was done. SDS-PAGE showed a single band for the enzyme. The results of this study
suggested that imidacloprid and lambda-cyhalothrin have significant inhibition effect on the activity of G6PD in in vitro. In
conclusion, lambda-cyhalothrin inhibits the enzyme activity more than imidacloprid