The objective of this study was to determine yield and quality characteristics of advanced generation cotton lines which developed by hybridization researches. Investigation was carried out in GAP International Agricultural Research and Training Center’s experiment area in 2013 and 2014 growing seasons. In the study, six advanced generation cotton lines and two control cotton varieties (Stoneville 468 and GW Teks) were used as materials. The experiments were arranged as a randomized complete block design with four replications. The results of statistical analysis indicated that there were significant differences among genotypes for yield and ginning percentage; both genotypes and year were significant for fiber length, fiber strength, elongation, uniformity; genotypes, years and genotypes x year interaction was significant for short fiber index, and finally it was detected that first picking percentage and fiber fineness significantly affected from year differences. According to results of this study it was determined that GW Teks, KP-24, 2/2 and 6/1 genotypes showed higher values and shared similar statistical groups for seed cotton yield, 30/4 and Stoneville 468 for ginning percentage. All genotypes classified in the same group except Stoneville 468 for fiber length. GW Teks (control variety) had higher values in terms of fiber strength, uniformity and short fiber index, while Stoneville 468 and 2/2 genotypes had higher values for elongation