Exploratory Animal and Medical Research, West Bengal Veterinary Alumni Association
Abstract
PCR-SSCP analysis was carried out to investigate the polymorphism of prolactin gene in Haringhata Black
chicken. The amplified fragment (497 bp) of promoter region of prolactin gene revealed three types of SSCP banding
pattern arbitrarily assigned as CC, CD and DD genotypes on 10 percent polyacrylamide gel. The heterozygous CD genotypes
showed presence of maximum five bands while rest two homozygous genotypes CC and DD showed three and two bands,
respectively. In the present study, genotype frequencies were found to be 0.622 for CC, 0.341 for CD and 0.037 for DD
genotype with respect to promoter region of prolactin gene. The frequencies of C and D alleles were estimated as 0.793
and 0.207, respectively. The calculated Chi square value revealed that the population was in Hardy Weinberg Equilibrium
with respect to promoter region of prolactin gene in Haringhata Black Chicken