Removal characteristics of tetracyclines resistant bacteria and tetracycline resistance genes in sludge deep dehydration

Abstract

In order to investigate the removal characteristics of Tetracyclines Resistant Bacteria (TRB) and Tetracycline Resistance Genes (TRGs) in sludge deep dehydration,Dilution plate coating method and Real-Time Polymerase chain reaction (RT-PCR) were used to detected the numbers of TRB and four tet genes (tetA、tetB、tetM and tetX) abundant before and after sludge dehydration,and then TRB and TRGs removal characteristics before and after sludge deep dehydration were analyzed.Results showed that:both TRB and TRGs were determined before and after sludge deep dehydration.However,the TRB and TRGs were partly removed after the treatment of sludge deep dehydration.tetA, tetB and tetM removal percentages reached more than 80%.The results can be used as reference for the control of TRB and TRGs contamination