Reaserch on temperature-sensitive regulation mechanism of transcription factor TDF1 to thermo-sensitive male sterile mutant <i>atms1</i> of <i>arabidopsis thaliana</i>

Abstract

TDF1 is a transcription factor of MYB family.It is involved in the process of callose degradation and regulating the tapetum differentiation,thus affecting the normal development of pollen.This paper investigates the temperature-sensitive mechanism of an Arabidopsis male sterile mutant atms1 (ambient temperature-sensory male steility1) obtained by screening in the progeny generations of wild-type Col-0 mutagenized by EMS. TDF1 Antisense RNA vector was introduced into atms1 background to inhibit the expression of TDF1 to observe the changes of atms1 mutant pollen fertility.We found that the fertility was restored at 27 ℃.The results show that TDF1 may have regulatory effects on ATMS1m in the process of pollen development