Background
& Objective: Nanomedicine delivery systems are known
as potent therapeutictools. In addition to possessing an effective agent of
turmeric, Curcumin shows antibacterial properties as well. Curcumin
is not water-soluble and it can be solved in water via nanotechnology-base methods.
Chitosan is a natural and biodegradable substance that is utilized for the Production
of Nanoparticles (NPs) carrying drug. In the following investigation, curcumin is
loaded in chitosan NPs and ultimately, the resulting nano-drug is studied as an
antibacterial agent. Materials & Methods: In
this study, NPs are produced using chitosan and Tripolyphosphate (TPP) salt. Curcumin solution was loaded in chitosan
NPs during their production. Next, the skins of BALB/c mice infected with staphylococcus
aureus are treated by curcumin-loaded chitosan NPs for 3 days. Afterwards,
in order to evaluate the antibacterial property of the nano-drug, these skin
suspensions of mice are cultured in bacterial medium. Results:
Dynamic
Light Scattering (DLS) reveals the charge of + 7 ± 2 mV and
the size of 160 ± 10 nm in curcumin-loaded chitosan NPs. Moreover, Transmission
electron microscopy (TEM) and atomic force microscopy (AFM) indicates
a spiral shape. Therefore, the evaluation of the optical density by
spectrophotometry demonstrates that 75 ± 2 % of curcumin are loaded in chitosan
NPs. Bacterial culture shows that curcumin-loaded chitosan NPs significantly
inhibited staphylococcus aureus growth. Conclusion: This
study demonstrates that curcumin-loaded
chitosan NPs can be applied as a potent agent in treatment of bacterial skin infections
