Effects of Artemisia lanata Extract on Reproductive Parameters of Female Rats

Abstract

Objective: Until date, there is no report on safety of Artemisia lanata. This study aimed to determine the possible undesirable effects of A. lanata on reproduction of female rats. Materials and Methods: The pregnant rats were treated (i.p.) with vehicle or 200 and 400mg/kg of A. lanata hydroalcoholic extract from the 2-8 day of pregnancy. Then, number and weight of neonates, duration of pregnancy, and percent of dead fetuses were determined. Furthermore, cytotoxicity of this plant was tested using fibroblast (L929) and Chinese hamster ovary (Cho) cell lines. Results: The A. lanata had no significant effect on duration of pregnancy, average number of neonates, and weight of neonates. However, administration of 200 and 400 mg/kg of the extract led to 30 and 44% abortion in animals, respectively. The extract at concentrations ≥ 200 μg/ml significantly (P < 0.001) inhibited the proliferation of L929 fibroblast cells. Regarding the Cho cells, the extract induced toxicity only at concentration of 800 μg/ml (P < 0.010). Conclusion: Our results showed that continuous consumption of A. lanata in pregnancy may increase the risk of abortion and also may have toxic effect on some cells