In this research, anatomical characteristics of peduncle and of epidermis in eight populations of Trigonella disperma in Iran were studied. Various anatomical characters were compared such as number of collenchyma, parenchyma, sclerenchyma fiber layers, and number of vascular bundles, density and size of stomata in adaxial and abaxial surfaces of leaflet, stomata type and epidermal cell shape. A variety of noticeable anatomical features were seen in the studied populations. Obtained results were analyzed by means of MVSP ver3.1 which attest intraspecific variations